Cloning and functional expression of human cDNA for the ETB endothelin receptor

Aiji Sakamoto; Masashi Yanagisawa; Takeshi Sakurai; Yoh Takuwa; Hiromi Yanagisawa; Tomoh Masaki

doi:10.1016/0006-291X(91)90158-4

Cloning and functional expression of human cDNA for the ET_B endothelin receptor

Aiji Sakamoto, Masashi Yanagisawa, Takeshi Sakurai, Yoh Takuwa, Hiromi Yanagisawa, Tomoh Masaki

Research output: Contribution to journal › Article › peer-review

271 Scopus citations

Abstract

We report the cloning of human cDNA encoding an ET_B (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ET_B endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ET_B and ET_A receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca²⁺]_i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing ¹²⁵I-labeled endothelin-1 binding and in eliciting [Ca²⁺]_i transients. The ET_B receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo.

Original language	English (US)
Pages (from-to)	656-663
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	178
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(91)90158-4
State	Published - Jul 31 1991

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(91)90158-4

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title = "Cloning and functional expression of human cDNA for the ETB endothelin receptor",

abstract = "We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo.",

author = "Aiji Sakamoto and Masashi Yanagisawa and Takeshi Sakurai and Yoh Takuwa and Hiromi Yanagisawa and Tomoh Masaki",

note = "Funding Information: Foundation, the University of Tsukuba Project Research, and the Ministry and Culture of Japan.",

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T1 - Cloning and functional expression of human cDNA for the ETB endothelin receptor

AU - Sakamoto, Aiji

AU - Yanagisawa, Masashi

AU - Sakurai, Takeshi

AU - Takuwa, Yoh

AU - Yanagisawa, Hiromi

AU - Masaki, Tomoh

N1 - Funding Information: Foundation, the University of Tsukuba Project Research, and the Ministry and Culture of Japan.

PY - 1991/7/31

Y1 - 1991/7/31

N2 - We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo.

AB - We report the cloning of human cDNA encoding an ETB (non-isopeptide-selective) subtype of the endothelin receptor. The predicted amino acid sequence of the human ETB endothelin receptor was 87.8% and 62.9% identical with the previously cloned rat ETB and ETA receptors, respectively. COS cells transiently transfected with the cloned cDNA expressed specific, high-affinity binding sites for endothelin isopeptides and responded to the peptides with a transient increase of [Ca2+]i; endothelin-1 and endothelin-3 exhibited approximately equal potencies both in displacing 125I-labeled endothelin-1 binding and in eliciting [Ca2+]i transients. The ETB receptor mRNAs were expressed in various human tissues and also in the intact porcine aortic intimal cells ex vivo.

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JO - Biochemical and Biophysical Research Communications

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Cloning and functional expression of human cDNA for the ET_B endothelin receptor

Abstract

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