Classification of PDZ domains

Ilya Bezprozvanny; Anton Maximov

doi:10.1016/S0014-5793(01)03214-8

Classification of PDZ domains

Ilya Bezprozvanny, Anton Maximov

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

A diverse family of PDZ domains has been identified, but the rules that govern their ligand specificity are not clear. Here we propose a novel classification of PDZ domains based on the nature of amino acids in the two critical positions in the PDZ domain fold. Using these principles, we classified PDZ domains present in the SMART database. Using yeast two-hybrid, in vitro pull-down and plasmon surface resonance assays, we demonstrated that in agreement with their position in the proposed classification the Mint1-1, hINADL-5, and PAR6 PDZ domains display similar dual ligand specificity. The proposed classification helps to organize PDZ domain containing proteins.

Original language	English (US)
Pages (from-to)	457-462
Number of pages	6
Journal	FEBS Letters
Volume	509
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)03214-8
State	Published - Dec 14 2001

Keywords

INADL
Mint1
PAR6
PDZ domain
Primary sequence analysis
Sequence database search

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(01)03214-8

Cite this

@article{f3ba5bf0060e4afb8d03a594fad7d134,

title = "Classification of PDZ domains",

abstract = "A diverse family of PDZ domains has been identified, but the rules that govern their ligand specificity are not clear. Here we propose a novel classification of PDZ domains based on the nature of amino acids in the two critical positions in the PDZ domain fold. Using these principles, we classified PDZ domains present in the SMART database. Using yeast two-hybrid, in vitro pull-down and plasmon surface resonance assays, we demonstrated that in agreement with their position in the proposed classification the Mint1-1, hINADL-5, and PAR6 PDZ domains display similar dual ligand specificity. The proposed classification helps to organize PDZ domain containing proteins.",

keywords = "INADL, Mint1, PAR6, PDZ domain, Primary sequence analysis, Sequence database search",

author = "Ilya Bezprozvanny and Anton Maximov",

note = "Funding Information: We are thankful to Nick Grishin and Thomas C. S{\"u}dhof for helpful discussions and to Sally Waard for help with and access to BIAcore 2000. I.B. is thankful to D. Brutlag (Stanford University) for teaching superb course in bioinformatics. Supported by Robert A. Welch Foundation and the National Institute of Health (NS38082 and NS39552).",

year = "2001",

month = dec,

day = "14",

doi = "10.1016/S0014-5793(01)03214-8",

language = "English (US)",

volume = "509",

pages = "457--462",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Classification of PDZ domains

AU - Bezprozvanny, Ilya

AU - Maximov, Anton

N1 - Funding Information: We are thankful to Nick Grishin and Thomas C. Südhof for helpful discussions and to Sally Waard for help with and access to BIAcore 2000. I.B. is thankful to D. Brutlag (Stanford University) for teaching superb course in bioinformatics. Supported by Robert A. Welch Foundation and the National Institute of Health (NS38082 and NS39552).

PY - 2001/12/14

Y1 - 2001/12/14

N2 - A diverse family of PDZ domains has been identified, but the rules that govern their ligand specificity are not clear. Here we propose a novel classification of PDZ domains based on the nature of amino acids in the two critical positions in the PDZ domain fold. Using these principles, we classified PDZ domains present in the SMART database. Using yeast two-hybrid, in vitro pull-down and plasmon surface resonance assays, we demonstrated that in agreement with their position in the proposed classification the Mint1-1, hINADL-5, and PAR6 PDZ domains display similar dual ligand specificity. The proposed classification helps to organize PDZ domain containing proteins.

AB - A diverse family of PDZ domains has been identified, but the rules that govern their ligand specificity are not clear. Here we propose a novel classification of PDZ domains based on the nature of amino acids in the two critical positions in the PDZ domain fold. Using these principles, we classified PDZ domains present in the SMART database. Using yeast two-hybrid, in vitro pull-down and plasmon surface resonance assays, we demonstrated that in agreement with their position in the proposed classification the Mint1-1, hINADL-5, and PAR6 PDZ domains display similar dual ligand specificity. The proposed classification helps to organize PDZ domain containing proteins.

KW - INADL

KW - Mint1

KW - PAR6

KW - PDZ domain

KW - Primary sequence analysis

KW - Sequence database search

UR - http://www.scopus.com/inward/record.url?scp=0035861856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035861856&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(01)03214-8

DO - 10.1016/S0014-5793(01)03214-8

M3 - Article

C2 - 11749973

AN - SCOPUS:0035861856

SN - 0014-5793

VL - 509

SP - 457

EP - 462

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Classification of PDZ domains

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this