TY - JOUR
T1 - Circadian regulation of lodopsin gene expression in embryonic photoreceptors in retinal cell culture
AU - Pierce, Mary E.
AU - Sheshberadaran, Hooshmand
AU - Zhe Zhang, Zhang
AU - Fox, Lyle E.
AU - Applebury, Meredithe L.
AU - Takahashi, Joseph S.
N1 - Funding Information:
The authors wish to thank Ms. D. Barker for technical assistance, Dr. J. C. Besharse for support while writing this manuscript, Drs. G. Cahill and C. Green for comments on this manuscript, Dr. D. Engel for pCH3dR8, used to generate histone probes, Dr. Tiansen Li for help in screening libraries, and Dr. J. Nathans for clone hs.7, used in the initial screening. This work was supported by National Eye Institute grants EYOEl67 to M. E. P., EY08467 to J. S. T., and EYO4801 to M. L. A. and by an NSF Center for Biological Timing grant to J. S. T.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/4
Y1 - 1993/4
N2 - A circadian clock regulates a number of diverse physiological functions in the vertebrate eye. In this study, we show that mRNA for the red-sensitive cone pigment, iodopsin, fluctuates with a circadian rhythm in chicken retina. Transcript levels increase in the late afternoon just prior to the time of cone disc shedding. Furthermore, iodopsin mRNA levels are regulated similarly by a circadian oscillator in primary cultures of dispersed embryonic chick retina. Nuclear run-on experiments show that the circadian regulation of iodopsin transcript abundance occurs at the level of gene transcription. Our results provide a demonstration of clock-regulated gene expression in a vertebrate preparation maintained in cell culture.
AB - A circadian clock regulates a number of diverse physiological functions in the vertebrate eye. In this study, we show that mRNA for the red-sensitive cone pigment, iodopsin, fluctuates with a circadian rhythm in chicken retina. Transcript levels increase in the late afternoon just prior to the time of cone disc shedding. Furthermore, iodopsin mRNA levels are regulated similarly by a circadian oscillator in primary cultures of dispersed embryonic chick retina. Nuclear run-on experiments show that the circadian regulation of iodopsin transcript abundance occurs at the level of gene transcription. Our results provide a demonstration of clock-regulated gene expression in a vertebrate preparation maintained in cell culture.
UR - http://www.scopus.com/inward/record.url?scp=0027245622&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027245622&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(93)90161-J
DO - 10.1016/0896-6273(93)90161-J
M3 - Article
C2 - 8476610
AN - SCOPUS:0027245622
SN - 0896-6273
VL - 10
SP - 579
EP - 584
JO - Neuron
JF - Neuron
IS - 4
ER -