@article{81e9e03b4fe04a7bb569615816bee2eb,
title = "Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating",
abstract = "Cilia beating is powered by the inner and outer dynein arms (IDAs and ODAs). These multi-subunit macrocomplexes are arranged in two rows on each outer doublet along the entire cilium length, except its distal end. To generate cilia beating, the activity of ODAs and IDAs must be strictly regulated locally by interactions with the dynein arm-associated structures within each ciliary unit and coordinated globally in time and space between doublets and along the axoneme. Here, we provide evidence of a novel ciliary complex composed of two conserved WD-repeat proteins, Fap43p and Fap44p. This complex is adjacent to another WD-repeat protein, Fap57p, and most likely the two-headed inner dynein arm, IDA I1. Loss of either protein results in altered waveform, beat stroke and reduced swimming speed. The ciliary localization of Fap43p and Fap44p is interdependent in the ciliate Tetrahymena thermophila.",
keywords = "Dyh6, Dyh7, Tether/tether head complex, Wdr52, Wdr65, Wdr96",
author = "Paulina Urbanska and Ewa Joachimiak and Rafa{\l} Bazan and Gang Fu and Martyna Poprzeczko and Hanna Fabczak and Daniela Nicastro and Dorota Wloga",
note = "Funding Information: We are very grateful to the following persons, who helped us in this research: Henryk Bilski and Szymon Suski from the Laboratory of Electron Microscopy of the Nencki Institute; Tytus Bernas, Artur Wolny and Malgorzata Calka, the members of the Laboratory of Imaging Tissue Structure and Function of the Nencki Institute, for their help in visualization of cilia beating; Dr. Jacek Gaertig, for providing the plasmid containing a coding region of Tetrahymena codon optimized BirA*; Dr. Tokuko Haraguchi, for providing the plasmid with the pur4 cassette; and Dr. Kazufumi Mochizuki, for providing the pMcoDel vector. The mass spectrometry analyses were performed in the Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland. The monoclonal anti-?-tubulin 12G10 antibody, developed by J. Frankel and E. M. Nelsen, was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology, Iowa City, IA 52242. This research was supported by the following grants: Polish Ministry of Science and Higher Education Grant no. N301706640, National Science Centre, Poland 2014/14/M/NZ3/00511 (Harmonia 6) grant and EMBO Installation Grant no. 2331 to DW; National Science Centre, Poland 2014/13/N/NZ3/04612 Preludium 7 grant to PU; and National Institutes of Health award R01 GM083122 to DN. Funding Information: Acknowledgements We are very grateful to the following persons, who helped us in this research: Henryk Bilski and Szymon Suski from the Laboratory of Electron Microscopy of the Nencki Institute; Tytus Bernas, Artur Wolny and Malgorzata Calka, the members of the Laboratory of Imaging Tissue Structure and Function of the Nencki Institute, for their help in visualization of cilia beating; Dr. Jacek Gaer-tig, for providing the plasmid containing a coding region of Tetrahymena codon optimized BirA*; Dr. Tokuko Haraguchi, for providing the plasmid with the pur4 cassette; and Dr. Kazufumi Mochizuki, for providing the pMcoDel vector. The mass spectrometry analyses were performed in the Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland. The monoclonal anti-α-tubulin 12G10 antibody, developed by J. Frankel and E. M. Nelsen, was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology, Iowa City, IA 52242. This research was supported by the following grants: Polish Ministry of Science and Higher Education Grant no. N301706640, National Science Centre, Poland 2014/14/M/NZ3/00511 (Harmonia 6) grant and EMBO Installation Grant no. 2331 to DW; National Science Centre, Poland 2014/13/N/NZ3/04612 Preludium 7 grant to PU; and National Institutes of Health award R01 GM083122 to DN. Publisher Copyright: {\textcopyright} 2018, The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1007/s00018-018-2819-7",
language = "English (US)",
volume = "75",
pages = "4479--4493",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-682X",
publisher = "Birkhauser Verlag Basel",
number = "24",
}