Chromosomal loop anchorage sites appear to be evolutionarily conserved

Peter N. Cockerill; William T. Garrard

doi:10.1016/0014-5793(86)81377-1

Chromosomal loop anchorage sites appear to be evolutionarily conserved

Peter N. Cockerill, William T. Garrard

Molecular Biology

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

We have previously identified a class of DNA sequence elements, termed matrix association regions (MARs), which specifically bind to nuclear matrices in vitro and are believed to be at the bases of chromosomal loops in vivo [1]. Here we demonstrate that nuclear matrices prepared from the yeast Saccharomyces cerevisiae will specifically bind an MAR sequence derived from the mouse kappa light chain immunoglobulin gene. This suggests that both MAR sequences and their binding sites have been strongly evolutionarily conserved.

Original language	English (US)
Pages (from-to)	5-7
Number of pages	3
Journal	FEBS Letters
Volume	204
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)81377-1
State	Published - Aug 11 1986

Keywords

(Yeast)
Chromatin loop
Enhancer
Immunoglobulin gene
Nuclear matrix
Nuclear scaffold
Topoisomerase II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)81377-1

Cite this

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title = "Chromosomal loop anchorage sites appear to be evolutionarily conserved",

abstract = "We have previously identified a class of DNA sequence elements, termed matrix association regions (MARs), which specifically bind to nuclear matrices in vitro and are believed to be at the bases of chromosomal loops in vivo [1]. Here we demonstrate that nuclear matrices prepared from the yeast Saccharomyces cerevisiae will specifically bind an MAR sequence derived from the mouse kappa light chain immunoglobulin gene. This suggests that both MAR sequences and their binding sites have been strongly evolutionarily conserved.",

keywords = "(Yeast), Chromatin loop, Enhancer, Immunoglobulin gene, Nuclear matrix, Nuclear scaffold, Topoisomerase II",

author = "Cockerill, {Peter N.} and Garrard, {William T.}",

note = "Funding Information: We are grateful to J. Allen for yeast nuclear matrices,D .S. Gross for review and M. Rotondi for preparationo f this manuscript.T his work was supported by grants GM2201, GM29935, and GM31689 from NIH and grant I-823 from The Robert A. Welch Foundation.",

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T1 - Chromosomal loop anchorage sites appear to be evolutionarily conserved

AU - Cockerill, Peter N.

AU - Garrard, William T.

N1 - Funding Information: We are grateful to J. Allen for yeast nuclear matrices,D .S. Gross for review and M. Rotondi for preparationo f this manuscript.T his work was supported by grants GM2201, GM29935, and GM31689 from NIH and grant I-823 from The Robert A. Welch Foundation.

PY - 1986/8/11

Y1 - 1986/8/11

N2 - We have previously identified a class of DNA sequence elements, termed matrix association regions (MARs), which specifically bind to nuclear matrices in vitro and are believed to be at the bases of chromosomal loops in vivo [1]. Here we demonstrate that nuclear matrices prepared from the yeast Saccharomyces cerevisiae will specifically bind an MAR sequence derived from the mouse kappa light chain immunoglobulin gene. This suggests that both MAR sequences and their binding sites have been strongly evolutionarily conserved.

AB - We have previously identified a class of DNA sequence elements, termed matrix association regions (MARs), which specifically bind to nuclear matrices in vitro and are believed to be at the bases of chromosomal loops in vivo [1]. Here we demonstrate that nuclear matrices prepared from the yeast Saccharomyces cerevisiae will specifically bind an MAR sequence derived from the mouse kappa light chain immunoglobulin gene. This suggests that both MAR sequences and their binding sites have been strongly evolutionarily conserved.

KW - (Yeast)

KW - Chromatin loop

KW - Enhancer

KW - Immunoglobulin gene

KW - Nuclear matrix

KW - Nuclear scaffold

KW - Topoisomerase II

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JO - FEBS Letters

JF - FEBS Letters

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Chromosomal loop anchorage sites appear to be evolutionarily conserved

Abstract

Keywords

ASJC Scopus subject areas

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