TY - JOUR
T1 - Chinese hamster × mouse hybrid cells segregating mouse chromosomes and isozymes
AU - Minna, John D.
AU - Marshall, Thomas H.
AU - Shaffer-Berman, Patricia V.
PY - 1975/10/1
Y1 - 1975/10/1
N2 - Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.
AB - Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and one pair (PGD-PGM2) to be syntenic. This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.
UR - http://www.scopus.com/inward/record.url?scp=0016567973&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016567973&partnerID=8YFLogxK
U2 - 10.1007/BF01538667
DO - 10.1007/BF01538667
M3 - Article
C2 - 1235911
AN - SCOPUS:0016567973
SN - 0098-0366
VL - 1
SP - 355
EP - 369
JO - Somatic Cell Genetics
JF - Somatic Cell Genetics
IS - 4
ER -