TY - JOUR
T1 - Chararacterization of G-protein α subunits in the Gq class
T2 - Expression in murine tissues and in stromal and hematopoietic cell lines
AU - Wilkie, Thomas M.
AU - Scherle, Peggy A.
AU - Strathmann, Michael P.
AU - Slepak, Vladlen Z.
AU - Simon, Melvin I.
PY - 1991
Y1 - 1991
N2 - Murine Gα14 and Gα15 cDNAs encode distinct α subunits of heterotrimeric guanine nucleotide-binding poteins (G proteins). These α subunits are related to members of the Gq class and share certain sequence characteristics with Gαq Gα11, and Gα16, such as the absence of a pertussis toxin ADP-ribosylation site. Gα11 and Gαq are ubiquitously expressed among murine tissues but α14 is predominantly expressed in spleen, lung, kidney, and testis whereas Gα15 is pimarily restricted to hematopoietic lineages. Among hematopotetic cell lines, Gα11 mRNA is found in all cell lines tested, Gαq is expressed widely but is not found in most T-cell lines, Gα15 is predominantly expressed in myeloid and B-cell lineages, and Gα14 is expressed in bone marrow adherent (stromal) cells, certain early myeloid cells, and progenitor B cells. Polyclonal antisera produced from synthetic peptides that correspond to two regions of Gα15 react with a protein of 42 kDA expressed in B-cell membranes and in Escherichia coli transformed with Gα15 cDNA. The expression patterns that were observed in mouse tissues and cell lines indicate that each of the α subunits in the Gq class may be involved in pertussis toxin-insensitive signal-transduction pathways that are fundamental to hematopoietic cell differentiation and function.
AB - Murine Gα14 and Gα15 cDNAs encode distinct α subunits of heterotrimeric guanine nucleotide-binding poteins (G proteins). These α subunits are related to members of the Gq class and share certain sequence characteristics with Gαq Gα11, and Gα16, such as the absence of a pertussis toxin ADP-ribosylation site. Gα11 and Gαq are ubiquitously expressed among murine tissues but α14 is predominantly expressed in spleen, lung, kidney, and testis whereas Gα15 is pimarily restricted to hematopoietic lineages. Among hematopotetic cell lines, Gα11 mRNA is found in all cell lines tested, Gαq is expressed widely but is not found in most T-cell lines, Gα15 is predominantly expressed in myeloid and B-cell lineages, and Gα14 is expressed in bone marrow adherent (stromal) cells, certain early myeloid cells, and progenitor B cells. Polyclonal antisera produced from synthetic peptides that correspond to two regions of Gα15 react with a protein of 42 kDA expressed in B-cell membranes and in Escherichia coli transformed with Gα15 cDNA. The expression patterns that were observed in mouse tissues and cell lines indicate that each of the α subunits in the Gq class may be involved in pertussis toxin-insensitive signal-transduction pathways that are fundamental to hematopoietic cell differentiation and function.
KW - Heterotrimeric GTP-binding protein
KW - Multigene family
KW - Phospholipase C
KW - Signal transduction
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U2 - 10.1073/pnas.88.22.10049
DO - 10.1073/pnas.88.22.10049
M3 - Article
C2 - 1946421
AN - SCOPUS:0025836752
SN - 0027-8424
VL - 88
SP - 10049
EP - 10053
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 22
ER -