Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney; Gregg B. Fields; Samuel Achilefu; W. Barry Edwards

doi:10.1117/12.810096

Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Philip P. Cheney, Gregg B. Fields, Samuel Achilefu, W. Barry Edwards

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

Original language	English (US)
Title of host publication	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
DOIs	https://doi.org/10.1117/12.810096
State	Published - 2009
Externally published	Yes
Event	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications - San Jose, CA, United States Duration: Jan 26 2009 → Jan 29 2009

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7190
ISSN (Print)	1605-7422

Conference

Conference	Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications
Country/Territory	United States
City	San Jose, CA
Period	1/26/09 → 1/29/09

Keywords

Cancer
Fluorescence
Gelatinase
MMP
Optical imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.810096

Cite this

Cheney, P. P., Fields, G. B., Achilefu, S., & Edwards, W. B. (2009). Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. In Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications [719011] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7190). https://doi.org/10.1117/12.810096

Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. / Cheney, Philip P.; Fields, Gregg B.; Achilefu, Samuel et al.
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications. 2009. 719011 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7190).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Cheney, PP, Fields, GB, Achilefu, S & Edwards, WB 2009, Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging. in Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications., 719011, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7190, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications, San Jose, CA, United States, 1/26/09. https://doi.org/10.1117/12.810096

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abstract = "The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.",

keywords = "Cancer, Fluorescence, Gelatinase, MMP, Optical imaging",

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doi = "10.1117/12.810096",

language = "English (US)",

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booktitle = "Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications",

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AU - Cheney, Philip P.

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AU - Achilefu, Samuel

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N2 - The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

AB - The prevalence of the gelatinases, MMP-2 and MMP-9, in many human tumors, including breast, colorectal, prostate and gastric cancer, make them an attractive target for molecular imaging. A self assembling homotrimeric triple helical peptide (THP), incorporating sequences from type V collagen with high specificity to MMP-2 and MMP-9, was previously developed. To investigate the viability of a THP for gelatinase imaging, we conjugated 5FAM to ε-amino groups of lysine flanking the hydrolysis site and subjected this substrate (THP-5FAM) to vitro analysis. The synthesis and in vitro results was presented.

KW - Cancer

KW - Fluorescence

KW - Gelatinase

KW - MMP

KW - Optical imaging

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications

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ER -

Characterization of quenched fluorescent triple helical peptides for MMP-2 and MMP-9 optical imaging

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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