TY - JOUR
T1 - Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry
AU - Hunt, Donald F.
AU - Henderson, Robert A.
AU - Shabanowitz, Jeffrey
AU - Sakaguchi, Kazuyasu
AU - Michel, Hanspeter
AU - Sevilir, Noelle
AU - Cox, Andrea L.
AU - Appella, Ettore
AU - Engelhard, Victor H.
PY - 1992
Y1 - 1992
N2 - Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.
AB - Antigens recognized by T cells are expressed as peptides bound to major histocompatibility complex (MHC) molecules. Microcapillary high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry was used to fractionate and sequence subpicomolar amounts of peptides isolated from the MHC molecule HLA-A2.1. Of 200 different species quantitated, eight were sequenced and four were found in cellular proteins. All were nine residues long and shared a distinct structural motif. The sensitivity and speed of this approach should enhance the analysis of peptides from small quantities of virally infected and transformed cells as well as those associated with autoimmune disease states.
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U2 - 10.1126/science.1546328
DO - 10.1126/science.1546328
M3 - Article
C2 - 1546328
AN - SCOPUS:0026507564
SN - 0036-8075
VL - 255
SP - 1261
EP - 1263
JO - Science
JF - Science
IS - 5049
ER -