TY - JOUR
T1 - Characterization of glucose transport system in Drosophila Kc cells
AU - Wang, May yun
AU - Wang, Chung
N1 - Funding Information:
Acknowledgemenfs~ We wish to thank Drs. Tzong-Hsiung Hseu and Bai-Ling Lin for comments on the manuscript. This work was supported by a grant (NSC 81-0203-B-001-14) from National Science Council of Taiwan, ROC.
PY - 1993/2/15
Y1 - 1993/2/15
N2 - We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki, values of 4 μM and 30 μM for cytochalasin B and phloretin, respectively. Moreover, phloridzin does not affect uptake of glucose by Kc cells. Thus, on the basis of inhibitor studies, it appears that this insect cell line contains glucose transporters similar to those of the mammalian cells. However, probes prepared from rat transporter cDNAs showed no cross-hybridization with Drosophila RNA under moderately stringent conditions. Sugar selectivity has also been investigated by competition assay. The km values for d-glucose, 2-deoxy-d-glucose, d-fructose and d-galactose are 2 mM, 6 mM, 17 mM and 16 mM, respectively. The results indicate that this transporter prefers d-glucose and that it does not discriminate between d-galactose and d-fructose.
AB - We have characterized the glucose transport system in the Kc cells of Drosophila melanogaster. The transporter is stereospecific and can be inhibited by both cytochalasin B and phloretin, with ki, values of 4 μM and 30 μM for cytochalasin B and phloretin, respectively. Moreover, phloridzin does not affect uptake of glucose by Kc cells. Thus, on the basis of inhibitor studies, it appears that this insect cell line contains glucose transporters similar to those of the mammalian cells. However, probes prepared from rat transporter cDNAs showed no cross-hybridization with Drosophila RNA under moderately stringent conditions. Sugar selectivity has also been investigated by competition assay. The km values for d-glucose, 2-deoxy-d-glucose, d-fructose and d-galactose are 2 mM, 6 mM, 17 mM and 16 mM, respectively. The results indicate that this transporter prefers d-glucose and that it does not discriminate between d-galactose and d-fructose.
KW - Drosophila melanogaster
KW - Glucose transporter
KW - Kc cell
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U2 - 10.1016/0014-5793(93)81284-7
DO - 10.1016/0014-5793(93)81284-7
M3 - Article
C2 - 7678820
AN - SCOPUS:0027447274
SN - 0014-5793
VL - 317
SP - 241
EP - 244
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -