Characterization of arrangement and expression of the beta-2 microglobulin locus in the sandbar and nurse shark

Hao Chen; Sarika Kshirsagar; Ingvill Jensen; Kevin Lau; Caitlin Simonson; Samuel F. Schluter

doi:10.1016/j.dci.2009.09.008

Characterization of arrangement and expression of the beta-2 microglobulin locus in the sandbar and nurse shark

Hao Chen, Sarika Kshirsagar, Ingvill Jensen, Kevin Lau, Caitlin Simonson, Samuel F. Schluter

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Beta 2 microglobulin (β2m) is an essential subunit of major histocompatibility complex (MHC) type I molecules. In this report, β2m cDNAs were identified and sequenced from sandbar shark spleen cDNA library. Sandbar shark β2m gene encodes one amino acid less than most teleost β2m genes, and 3 amino acids less than mammal β2m genes. Although sandbar shark β2m protein contains one β sheet less than that of human in the predicted protein structure, the overall structure of β2m proteins is conserved during evolution. Germline gene for the β2m in sandbar and nurse shark is present as a single locus. It contains three exons and two introns. CpG sites are evenly distributed in the shark β2m loci. Several DNA repeat elements were also identified in the shark β2m loci. Sequence analysis suggests that the β2m locus is not linked to the MHC I loci in the shark genome.

Original language	English (US)
Pages (from-to)	189-195
Number of pages	7
Journal	Developmental and Comparative Immunology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1016/j.dci.2009.09.008
State	Published - Feb 2010
Externally published	Yes

Keywords

Adaptive immune system
Beta 2 microglobulin
Evolution
Genomic arrangement
MHC

ASJC Scopus subject areas

Immunology
Developmental Biology

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10.1016/j.dci.2009.09.008

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title = "Characterization of arrangement and expression of the beta-2 microglobulin locus in the sandbar and nurse shark",

abstract = "Beta 2 microglobulin (β2m) is an essential subunit of major histocompatibility complex (MHC) type I molecules. In this report, β2m cDNAs were identified and sequenced from sandbar shark spleen cDNA library. Sandbar shark β2m gene encodes one amino acid less than most teleost β2m genes, and 3 amino acids less than mammal β2m genes. Although sandbar shark β2m protein contains one β sheet less than that of human in the predicted protein structure, the overall structure of β2m proteins is conserved during evolution. Germline gene for the β2m in sandbar and nurse shark is present as a single locus. It contains three exons and two introns. CpG sites are evenly distributed in the shark β2m loci. Several DNA repeat elements were also identified in the shark β2m loci. Sequence analysis suggests that the β2m locus is not linked to the MHC I loci in the shark genome.",

keywords = "Adaptive immune system, Beta 2 microglobulin, Evolution, Genomic arrangement, MHC",

author = "Hao Chen and Sarika Kshirsagar and Ingvill Jensen and Kevin Lau and Caitlin Simonson and Schluter, {Samuel F.}",

note = "Funding Information: This work was supported by Grant MCB-0621853 from the National Science Foundation to SFS . We thank Dr. Harris Bernstein for proofreading this manuscript. ",

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AU - Kshirsagar, Sarika

AU - Jensen, Ingvill

AU - Lau, Kevin

AU - Simonson, Caitlin

AU - Schluter, Samuel F.

N1 - Funding Information: This work was supported by Grant MCB-0621853 from the National Science Foundation to SFS . We thank Dr. Harris Bernstein for proofreading this manuscript.

PY - 2010/2

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N2 - Beta 2 microglobulin (β2m) is an essential subunit of major histocompatibility complex (MHC) type I molecules. In this report, β2m cDNAs were identified and sequenced from sandbar shark spleen cDNA library. Sandbar shark β2m gene encodes one amino acid less than most teleost β2m genes, and 3 amino acids less than mammal β2m genes. Although sandbar shark β2m protein contains one β sheet less than that of human in the predicted protein structure, the overall structure of β2m proteins is conserved during evolution. Germline gene for the β2m in sandbar and nurse shark is present as a single locus. It contains three exons and two introns. CpG sites are evenly distributed in the shark β2m loci. Several DNA repeat elements were also identified in the shark β2m loci. Sequence analysis suggests that the β2m locus is not linked to the MHC I loci in the shark genome.

AB - Beta 2 microglobulin (β2m) is an essential subunit of major histocompatibility complex (MHC) type I molecules. In this report, β2m cDNAs were identified and sequenced from sandbar shark spleen cDNA library. Sandbar shark β2m gene encodes one amino acid less than most teleost β2m genes, and 3 amino acids less than mammal β2m genes. Although sandbar shark β2m protein contains one β sheet less than that of human in the predicted protein structure, the overall structure of β2m proteins is conserved during evolution. Germline gene for the β2m in sandbar and nurse shark is present as a single locus. It contains three exons and two introns. CpG sites are evenly distributed in the shark β2m loci. Several DNA repeat elements were also identified in the shark β2m loci. Sequence analysis suggests that the β2m locus is not linked to the MHC I loci in the shark genome.

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KW - Beta 2 microglobulin

KW - Evolution

KW - Genomic arrangement

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Characterization of arrangement and expression of the beta-2 microglobulin locus in the sandbar and nurse shark

Abstract

Keywords

ASJC Scopus subject areas

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