TY - JOUR
T1 - Characterization of a monoclonal antibody reactive with a subset of human breast tumors
AU - Yuan, D.
AU - Hendler, F. J.
AU - Vitetta, E. S.
N1 - Funding Information:
1 Received May 26, 1981; accepted November 19, 1981. 2 Supported by Public Health Service grant CA-23115 from the National Cancer Institute (NCI); by the Ira Kassanoff Fund, The Blanche Mary Taxis Foundation; and by a Biomedical Research Support grant from The University of Texas Health Science Center at Dallas (UTHSCD). 3 Research procedures were in accord with the ethical standards of the Institutional Review Committee of UTIISCD. 4 Department of Microbiology, The University of Texas Health Science Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Tex. 75235. 5 Department of Internal Medicine, UTHSCD. 6Addressreprintrequests toDr. Hendler. 7We thank the following for providing us with cells: the NCI Breast Cancer Task Force Cell Culture Bank (Dr. C. B. Piczak, NCI, and Dr. E. Jensen, Mason Research Institute, Rockville, Md.); Dr. M. E. Lippman and Dr. W. R. Kidwell, NCI; Dr. R. G. Smith, Department of Medicine, and Dr. P. MacDonald and Dr. J. C. Porter, Department of Obstetrics, Gynecology, and Physiology, UTHSCD. We also thank Ms. E. Carlton, Ms. L. Trahan, Dr. D. Richardson, Ms. J. Tseng, and Mr. D. R. Quick, UTHSCD, for expert technical assistance; and Ms. G. A. Cheek and Ms. D. Marcoulides, UTHSCD, for secretarial assistance. We are also grateful to Dr. E. P. Frenkel, Department ofInternal Medicine, and Dr. Fred Silva, Department of Pathology, UTHSCD, and Dr. William Kingsley, Department of Pathology, Baylor Medical Center, Dallas, Tex., for their review of the scientific data. We are especially indebted to Dr. Jonathan W. Uhr, Department of Microbiology, UTHSCD, for his constant support and advice and his assistance with the manuscript.
PY - 1982/5
Y1 - 1982/5
N2 - Fusion of spleen cells, from mice immunized against the human hormone-dependent breast cancer cell line ZR-75-1, with mu rine myeloma cells resulted in the establishment of a hybridoma cell line, HY59-H10 (H59). The purified monoclonal IgM produced by the hybridoma reacts with the most differentiated human breast tumor cell lines but not with cells derived from normal breast secretions or with numerous other malignant cell lines. Of 106 biopsy specimens exam ined, H59 bound to 54% of malignant breast specimens, to 83% of fibroadenomas, and to 82% of specimens containing fibrocystic dis ease. Binding could not be detected in uninvolved breast tissue.
AB - Fusion of spleen cells, from mice immunized against the human hormone-dependent breast cancer cell line ZR-75-1, with mu rine myeloma cells resulted in the establishment of a hybridoma cell line, HY59-H10 (H59). The purified monoclonal IgM produced by the hybridoma reacts with the most differentiated human breast tumor cell lines but not with cells derived from normal breast secretions or with numerous other malignant cell lines. Of 106 biopsy specimens exam ined, H59 bound to 54% of malignant breast specimens, to 83% of fibroadenomas, and to 82% of specimens containing fibrocystic dis ease. Binding could not be detected in uninvolved breast tissue.
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U2 - 10.1093/jnci/68.5.719
DO - 10.1093/jnci/68.5.719
M3 - Article
C2 - 6279951
AN - SCOPUS:0019940190
SN - 0027-8874
VL - 68
SP - 719
EP - 728
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -