Chapter 24 Hippocampal grafts derived from embryonic trisomy 16 mice exhibit amyloid (A4) and neurofibrillary pathology

S. J. Richards; J. J. Waters; D. C. Rogers; F. L. Martel; D. R. Sparkman; C. L. White; K. Beyreuther; C. L. Masters; S. B. Dunnett

doi:10.1016/S0079-6123(08)62607-2

Chapter 24 Hippocampal grafts derived from embryonic trisomy 16 mice exhibit amyloid (A4) and neurofibrillary pathology

S. J. Richards, J. J. Waters, D. C. Rogers, F. L. Martel, D. R. Sparkman, C. L. White, K. Beyreuther, C. L. Masters, S. B. Dunnett

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Abstract

This chapter discusses the transplanted neocortical and hippocampal tissues derived from trisomic and control fetuses into the brains of normal recipient mice and monitored neuropathologcal changes occurring over 4–6 months within the grafts. In the experiments discussed in the chapter, embryos for transplantation were staged by vaginal plugs and the developmental age was confirmed by crown-rump length. Frontal cortex and hippocampal dissections were taken from 7 trisomic and 18 normal embryos assessed as E14-16. The trisomy 16 embryo was devoid of immunoreactivity to either antiserum. Neocortical and/or hippocampal sections derived from post mortem Alzheimer brain were included in each batch of immunocytochemical staining to confirm the activity and appropriate patterns of immunoreactivity of the antibodies. lmmunocytochemical staining with an anti-A4 antibody demonstrated the presence of deposits of A4 immunoreactivity around the cerebral vasculature within the trisomic grafts. The intracellular immunoreactivity of similar numbers of cells within trisomic grafts was observed with the A128 antiserum raised against purified paired helical filaments.

Original language	English (US)
Pages (from-to)	215-223
Number of pages	9
Journal	Progress in Brain Research
Volume	82
Issue number	C
DOIs	https://doi.org/10.1016/S0079-6123(08)62607-2
State	Published - Jan 1 1990

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Neuroscience(all)

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10.1016/S0079-6123(08)62607-2

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title = "Chapter 24 Hippocampal grafts derived from embryonic trisomy 16 mice exhibit amyloid (A4) and neurofibrillary pathology",

abstract = "This chapter discusses the transplanted neocortical and hippocampal tissues derived from trisomic and control fetuses into the brains of normal recipient mice and monitored neuropathologcal changes occurring over 4–6 months within the grafts. In the experiments discussed in the chapter, embryos for transplantation were staged by vaginal plugs and the developmental age was confirmed by crown-rump length. Frontal cortex and hippocampal dissections were taken from 7 trisomic and 18 normal embryos assessed as E14-16. The trisomy 16 embryo was devoid of immunoreactivity to either antiserum. Neocortical and/or hippocampal sections derived from post mortem Alzheimer brain were included in each batch of immunocytochemical staining to confirm the activity and appropriate patterns of immunoreactivity of the antibodies. lmmunocytochemical staining with an anti-A4 antibody demonstrated the presence of deposits of A4 immunoreactivity around the cerebral vasculature within the trisomic grafts. The intracellular immunoreactivity of similar numbers of cells within trisomic grafts was observed with the A128 antiserum raised against purified paired helical filaments.",

author = "Richards, {S. J.} and Waters, {J. J.} and Rogers, {D. C.} and Martel, {F. L.} and Sparkman, {D. R.} and White, {C. L.} and K. Beyreuther and Masters, {C. L.} and Dunnett, {S. B.}",

note = "Funding Information: The authors are grateful to Dr. Martin Bobrow and Mr John Crolla (Guy{\textquoteright}s Hospital, London) for making available the Robertsonian translocation mice strains and for continued advice regarding breeding regimes. Sincere thanks are also extended to Dr. Claude Wischik and Dr. Gerd Multhaup for their helpful discussions and Dr. David Mann for the provision of initial help on A4 immunohistochemistry. This work has been supported by the Medical Research Council and the Mental Health Foundation.",

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AU - Richards, S. J.

AU - Waters, J. J.

AU - Rogers, D. C.

AU - Martel, F. L.

AU - Sparkman, D. R.

AU - White, C. L.

AU - Beyreuther, K.

AU - Masters, C. L.

AU - Dunnett, S. B.

N1 - Funding Information: The authors are grateful to Dr. Martin Bobrow and Mr John Crolla (Guy’s Hospital, London) for making available the Robertsonian translocation mice strains and for continued advice regarding breeding regimes. Sincere thanks are also extended to Dr. Claude Wischik and Dr. Gerd Multhaup for their helpful discussions and Dr. David Mann for the provision of initial help on A4 immunohistochemistry. This work has been supported by the Medical Research Council and the Mental Health Foundation.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - This chapter discusses the transplanted neocortical and hippocampal tissues derived from trisomic and control fetuses into the brains of normal recipient mice and monitored neuropathologcal changes occurring over 4–6 months within the grafts. In the experiments discussed in the chapter, embryos for transplantation were staged by vaginal plugs and the developmental age was confirmed by crown-rump length. Frontal cortex and hippocampal dissections were taken from 7 trisomic and 18 normal embryos assessed as E14-16. The trisomy 16 embryo was devoid of immunoreactivity to either antiserum. Neocortical and/or hippocampal sections derived from post mortem Alzheimer brain were included in each batch of immunocytochemical staining to confirm the activity and appropriate patterns of immunoreactivity of the antibodies. lmmunocytochemical staining with an anti-A4 antibody demonstrated the presence of deposits of A4 immunoreactivity around the cerebral vasculature within the trisomic grafts. The intracellular immunoreactivity of similar numbers of cells within trisomic grafts was observed with the A128 antiserum raised against purified paired helical filaments.

AB - This chapter discusses the transplanted neocortical and hippocampal tissues derived from trisomic and control fetuses into the brains of normal recipient mice and monitored neuropathologcal changes occurring over 4–6 months within the grafts. In the experiments discussed in the chapter, embryos for transplantation were staged by vaginal plugs and the developmental age was confirmed by crown-rump length. Frontal cortex and hippocampal dissections were taken from 7 trisomic and 18 normal embryos assessed as E14-16. The trisomy 16 embryo was devoid of immunoreactivity to either antiserum. Neocortical and/or hippocampal sections derived from post mortem Alzheimer brain were included in each batch of immunocytochemical staining to confirm the activity and appropriate patterns of immunoreactivity of the antibodies. lmmunocytochemical staining with an anti-A4 antibody demonstrated the presence of deposits of A4 immunoreactivity around the cerebral vasculature within the trisomic grafts. The intracellular immunoreactivity of similar numbers of cells within trisomic grafts was observed with the A128 antiserum raised against purified paired helical filaments.

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Chapter 24 Hippocampal grafts derived from embryonic trisomy 16 mice exhibit amyloid (A4) and neurofibrillary pathology

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