Changes in morphology and optical properties of sclera and choroidal layers due to hyperosmotic agent

Raiyan T. Zaman, Narasimhan Rajaram, Brandon S. Nichols, Henry G. Rylander, Tianyi Wang, James W. Tunnell, Ashley J. Welch

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Light scattering in the normally white sclera prevents diagnostic imaging or delivery of a focused laser beam to a target in the underlying choroid layer. In this study, we examine optical clearing of the sclera and changes in blood flow resulting from the application of glycerol to the sclera of rabbits. Recovery dynamics are monitored after the application of saline. The speed of clearing for injection delivery is compared to the direct application of glycerol through an incision in the conjunctiva. Although, the same volume of glycerol was applied, the sclera cleared much faster (5 to 10 s) with the topical application of glycerol compared to the injection method (3 min). In addition, the direct topical application of glycerol spreads over a larger area in the sclera than the latter method. A diffuse optical spectroscopy system provided spectral analysis of the remitted light every two minutes during clearing and rehydration. Comparison of measurements to those obtained from phantoms with various absorption and scattering properties provided estimates of the absorption coefficient and reduced scattering coefficient of rabbit eye tissue.

Original languageEnglish (US)
Article number077008
JournalJournal of biomedical optics
Issue number7
StatePublished - Jul 2011
Externally publishedYes


  • Absorption coefficient
  • Diffuse optical spectroscopy
  • Hyperosmotic agents
  • Linear-probe
  • Optical clearing
  • Optical properties
  • Reduced scattering coefficient
  • Sclera

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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