Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis

Flora M. Vaccarino; Michael L. Schwartz; Rossana Raballo; Jon Nilsen; Julianne Rhee; Ming Zhou; Thomas Doetschman; J. Douglas Coffin; Jason J. Wyland; Yu Ting Elizabeth Hung

doi:10.1038/6350

Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis

Flora M. Vaccarino, Michael L. Schwartz, Rossana Raballo, Jon Nilsen, Julianne Rhee, Ming Zhou, Thomas Doetschman, J. Douglas Coffin, Jason J. Wyland, Yu Ting Elizabeth Hung

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Abstract

We show that fibroblast growth factor 2 (FGF2) and FGF receptors are transiently expressed by cells of the pseudostratified ventricular epithelium (PVE) during early neurogenesis. A single microinjection of FGF2 into cerebral ventricles of rat embryos at E15.5 increased the volume and total number of neurons in the adult cerebral cortex by 18% and 87%, respectively. Microinjection of FGF2 by the end of neurogenesis, at E20.5, selectively increased the number of glia. Mice lacking the FGF2 gene had fewer cortical neurons and gila at maturity. BrdU studies in FGF2-microinjected and FGF2- null animals suggested that FGF2 increases the proportion of dividing cells in the PVE without affecting the cell-cycle length. Thus, FGF2 increases the number of rounds of division of cortical progenitors.

Original language	English (US)
Pages (from-to)	246-253
Number of pages	8
Journal	Nature neuroscience
Volume	2
Issue number	3
DOIs	https://doi.org/10.1038/6350
State	Published - Mar 1999

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Neuroscience(all)

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@article{dd80eddd0b0b49aba743df4d7e09be1b,

title = "Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis",

abstract = "We show that fibroblast growth factor 2 (FGF2) and FGF receptors are transiently expressed by cells of the pseudostratified ventricular epithelium (PVE) during early neurogenesis. A single microinjection of FGF2 into cerebral ventricles of rat embryos at E15.5 increased the volume and total number of neurons in the adult cerebral cortex by 18% and 87%, respectively. Microinjection of FGF2 by the end of neurogenesis, at E20.5, selectively increased the number of glia. Mice lacking the FGF2 gene had fewer cortical neurons and gila at maturity. BrdU studies in FGF2-microinjected and FGF2- null animals suggested that FGF2 increases the proportion of dividing cells in the PVE without affecting the cell-cycle length. Thus, FGF2 increases the number of rounds of division of cortical progenitors.",

author = "Vaccarino, {Flora M.} and Schwartz, {Michael L.} and Rossana Raballo and Jon Nilsen and Julianne Rhee and Ming Zhou and Thomas Doetschman and Coffin, {J. Douglas} and Wyland, {Jason J.} and Hung, {Yu Ting Elizabeth}",

note = "Funding Information: This work was supported in part by grants from the NARSAD and the National Science Foundation (FMV) and NIH (MLS). The authors thank D. Stull and N. Sestan for help with in situ hybridization protocols and P. Rakic for comments on an earlier version of this manuscript. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1999",

month = mar,

doi = "10.1038/6350",

language = "English (US)",

volume = "2",

pages = "246--253",

journal = "Nature neuroscience",

issn = "1097-6256",

publisher = "Nature Publishing Group",

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T1 - Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis

AU - Vaccarino, Flora M.

AU - Schwartz, Michael L.

AU - Raballo, Rossana

AU - Nilsen, Jon

AU - Rhee, Julianne

AU - Zhou, Ming

AU - Doetschman, Thomas

AU - Coffin, J. Douglas

AU - Wyland, Jason J.

AU - Hung, Yu Ting Elizabeth

N1 - Funding Information: This work was supported in part by grants from the NARSAD and the National Science Foundation (FMV) and NIH (MLS). The authors thank D. Stull and N. Sestan for help with in situ hybridization protocols and P. Rakic for comments on an earlier version of this manuscript. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1999/3

Y1 - 1999/3

N2 - We show that fibroblast growth factor 2 (FGF2) and FGF receptors are transiently expressed by cells of the pseudostratified ventricular epithelium (PVE) during early neurogenesis. A single microinjection of FGF2 into cerebral ventricles of rat embryos at E15.5 increased the volume and total number of neurons in the adult cerebral cortex by 18% and 87%, respectively. Microinjection of FGF2 by the end of neurogenesis, at E20.5, selectively increased the number of glia. Mice lacking the FGF2 gene had fewer cortical neurons and gila at maturity. BrdU studies in FGF2-microinjected and FGF2- null animals suggested that FGF2 increases the proportion of dividing cells in the PVE without affecting the cell-cycle length. Thus, FGF2 increases the number of rounds of division of cortical progenitors.

AB - We show that fibroblast growth factor 2 (FGF2) and FGF receptors are transiently expressed by cells of the pseudostratified ventricular epithelium (PVE) during early neurogenesis. A single microinjection of FGF2 into cerebral ventricles of rat embryos at E15.5 increased the volume and total number of neurons in the adult cerebral cortex by 18% and 87%, respectively. Microinjection of FGF2 by the end of neurogenesis, at E20.5, selectively increased the number of glia. Mice lacking the FGF2 gene had fewer cortical neurons and gila at maturity. BrdU studies in FGF2-microinjected and FGF2- null animals suggested that FGF2 increases the proportion of dividing cells in the PVE without affecting the cell-cycle length. Thus, FGF2 increases the number of rounds of division of cortical progenitors.

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JO - Nature neuroscience

JF - Nature neuroscience

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ER -

Changes in cerebral cortex size are governed by fibroblast growth factor during embryogenesis

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