CEP120 interacts with CPAP and positively regulates centriole elongation

Yi Nan Lin, Chien Ting Wu, Yu Chih Lin, Wen Bin Hsu, Chieh Ju C. Tang, Ching Wen Chang, Tang K. Tang

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Centriole duplication begins with the formation of a single procentriole next to a preexisting centriole. CPAP (centrosomal protein 4.1-associated protein) was previously reported to participate in centriole elongation. Here, we show that CEP120 is a cell cycle-regulated protein that directly interacts with CPAP and is required for centriole duplication. CEP120 levels increased gradually from early S to G2/M and decreased significantly after mitosis. Forced overexpression of either CEP120 or CPAP not only induced the assembly of overly long centrioles but also produced atypical supernumerary centrioles that grew from these long centrioles. Depletion of CEP120 inhibited CPAP-induced centriole elongation and vice versa, implying that these proteins work together to regulate centriole elongation. Furthermore, CEP120 was found to contain an N-terminal microtubulebinding domain, a C-terminal dimerization domain, and a centriolar localization domain. Overexpression of a microtubule binding-defective CEP120-K76A mutant significantly suppressed the formation of elongated centrioles. Together, our results indicate that CEP120 is a CPAP-interacting protein that positively regulates centriole elongation.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalJournal of Cell Biology
Issue number2
StatePublished - Jul 2013
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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