Cell-free formation of RNA granules: Bound RNAs identify features and components of cellular assemblies

Tina W. Han; Masato Kato; Shanhai Xie; Leeju C. Wu; Hamid Mirzaei; Jimin Pei; Min Chen; Yang Xie; Jeffrey Allen; Guanghua Xiao; Steven L. McKnight

doi:10.1016/j.cell.2012.04.016

Cell-free formation of RNA granules: Bound RNAs identify features and components of cellular assemblies

Tina W. Han, Masato Kato, Shanhai Xie, Leeju C. Wu, Hamid Mirzaei, Jimin Pei, Min Chen, Yang Xie, Jeffrey Allen, Guanghua Xiao, Steven L. McKnight

Research output: Contribution to journal › Article › peer-review

602 Scopus citations

Abstract

Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 3′ UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly. PaperClip:

Original language	English (US)
Pages (from-to)	768-779
Number of pages	12
Journal	Cell
Volume	149
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2012.04.016
State	Published - May 11 2012

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2012.04.016

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title = "Cell-free formation of RNA granules: Bound RNAs identify features and components of cellular assemblies",

abstract = "Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 3′ UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly. PaperClip:",

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T1 - Cell-free formation of RNA granules

T2 - Bound RNAs identify features and components of cellular assemblies

AU - Han, Tina W.

AU - Kato, Masato

AU - Xie, Shanhai

AU - Wu, Leeju C.

AU - Mirzaei, Hamid

AU - Pei, Jimin

AU - Chen, Min

AU - Xie, Yang

AU - Allen, Jeffrey

AU - Xiao, Guanghua

AU - McKnight, Steven L.

PY - 2012/5/11

Y1 - 2012/5/11

N2 - Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 3′ UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly. PaperClip:

AB - Cellular granules lacking boundary membranes harbor RNAs and their associated proteins and play diverse roles controlling the timing and location of protein synthesis. Formation of such granules was emulated by treatment of mouse brain extracts and human cell lysates with a biotinylated isoxazole (b-isox) chemical. Deep sequencing of the associated RNAs revealed an enrichment for mRNAs known to be recruited to neuronal granules used for dendritic transport and localized translation at synapses. Precipitated mRNAs contain extended 3′ UTR sequences and an enrichment in binding sites for known granule-associated proteins. Hydrogels composed of the low complexity (LC) sequence domain of FUS recruited and retained the same mRNAs as were selectively precipitated by the b-isox chemical. Phosphorylation of the LC domain of FUS prevented hydrogel retention, offering a conceptual means of dynamic, signal-dependent control of RNA granule assembly. PaperClip:

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Cell-free formation of RNA granules: Bound RNAs identify features and components of cellular assemblies

Abstract

ASJC Scopus subject areas

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