Abstract
Cloned, neoplastic B cells (BCL1) have been used to evaluate the expression of the receptor for the B cell differentiation factor, BCDFμ. These cells do not secrete IgM before stimulation with BCDFμ-containing T cell supernatants (SN). By inducing cell cycle synchrony in this homogeneous population, the expression of the BCDFμ receptor could be evaluated as a function of the cell cycle. Responsiveness to BCDFμ-containing SN is maximal when the cells are in S and G2 phases of the cell cycle, and a 2-hr exposure of cells to BCDFμ-containing SN during S/G2 results in optimal IgM secretion 5 days later. Cells in S/G2 are also maximally effective in absorbing BCDFμ activity from SN. These data support the hypothesis that B cells do not respond to differentiative signals until after they are committed to at least one round of cell division.
Original language | English (US) |
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Pages (from-to) | 742-747 |
Number of pages | 6 |
Journal | Journal of Immunology |
Volume | 134 |
Issue number | 2 |
State | Published - 1985 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology