TY - JOUR
T1 - cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA
AU - Sakurai, Takeshi
AU - Yanagisawa, Masashi
AU - Inoue, Akihiro
AU - Ryan, Una S.
AU - Kimura, Sadao
AU - Mitsui, Youji
AU - Goto, Katsutoshi
AU - Masaki, Tomoh
N1 - Funding Information:
This work is supported in part by grants from the Ministry of Education, Science and Culture of Japan, and from the Uehara Memorial Foundation.
PY - 1991/2/28
Y1 - 1991/2/28
N2 - We report the cloning of a full-length cDNA encoding rat preproendothelin-1 (preproET-1). The predicted rat preproET-1 consists of 202 amino acid residues and highly similar to human, porcine and bovine preproET-1, respectively. The deduced 21-residue sequence of mature rat ET-1 is identical to human, porcine, canine and bovine ET-1. As in other mammalian species, the mature ET-1 is predicted to be produced from a 39-residue big ET-1 in the rat. Northern blot analysis showed that a single 2.3-kb preproET-1 mRNA is expressed not only in vascular endothelial cells but also in other rat tissues, including the lung, brain, uterus, stomach, heart, adrenal gland and kidney. These findings suggest that ET-1 may play roles as a local mediator in multiple organs both within and outside the cardiovascular system in the rat.
AB - We report the cloning of a full-length cDNA encoding rat preproendothelin-1 (preproET-1). The predicted rat preproET-1 consists of 202 amino acid residues and highly similar to human, porcine and bovine preproET-1, respectively. The deduced 21-residue sequence of mature rat ET-1 is identical to human, porcine, canine and bovine ET-1. As in other mammalian species, the mature ET-1 is predicted to be produced from a 39-residue big ET-1 in the rat. Northern blot analysis showed that a single 2.3-kb preproET-1 mRNA is expressed not only in vascular endothelial cells but also in other rat tissues, including the lung, brain, uterus, stomach, heart, adrenal gland and kidney. These findings suggest that ET-1 may play roles as a local mediator in multiple organs both within and outside the cardiovascular system in the rat.
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U2 - 10.1016/S0006-291X(05)81197-0
DO - 10.1016/S0006-291X(05)81197-0
M3 - Article
C2 - 1840483
AN - SCOPUS:0026084911
SN - 0006-291X
VL - 175
SP - 44
EP - 47
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -