Cations decrease specific [3H]-spiroperidol binding in human prefrontal cortex

Emina H. Huang; John M. Pickett; Joel D. Siegal; Anne C. Andorn

doi:10.1016/0024-3205(86)90469-8

Cations decrease specific [³H]-spiroperidol binding in human prefrontal cortex

Emina H. Huang, John M. Pickett, Joel D. Siegal, Anne C. Andorn

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Ligand binding at many physiologically relevant receptors is regulated by divalent cations. To determine whether [³H]-spiroperidol binding sites in prefrontal cortex might be physiologically relevant receptors, we examined the effect of ions on the binding of this ligand in postmortem human prefrontal cortex. Our results indicate that several cations decreased [³H]-spiroperidol binding in a dose-dependent fashion. Of these, Cd⁺⁺ and Zn⁺⁺ were the most able to decrease [³H]-spiroperidol binding with IC₅₀ of 5.5.5 +/- 2.4 × 10^-6M and 5.6 +/- 1.1 × 10^-5M respectively. These findings indicate that [³H]-spiroperidol may bind at physiologically relevant receptors in human prefrontal cortex.

Original language	English (US)
Pages (from-to)	1369-1373
Number of pages	5
Journal	Life Sciences
Volume	38
Issue number	15
DOIs	https://doi.org/10.1016/0024-3205(86)90469-8
State	Published - Apr 14 1986
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(86)90469-8

Cite this

@article{a2dd44bab4624a769c0582e57bdb6d6f,

title = "Cations decrease specific [3H]-spiroperidol binding in human prefrontal cortex",

abstract = "Ligand binding at many physiologically relevant receptors is regulated by divalent cations. To determine whether [3H]-spiroperidol binding sites in prefrontal cortex might be physiologically relevant receptors, we examined the effect of ions on the binding of this ligand in postmortem human prefrontal cortex. Our results indicate that several cations decreased [3H]-spiroperidol binding in a dose-dependent fashion. Of these, Cd++ and Zn++ were the most able to decrease [3H]-spiroperidol binding with IC50 of 5.5.5 +/- 2.4 × 10-6M and 5.6 +/- 1.1 × 10-5M respectively. These findings indicate that [3H]-spiroperidol may bind at physiologically relevant receptors in human prefrontal cortex.",

author = "Huang, {Emina H.} and Pickett, {John M.} and Siegal, {Joel D.} and Andorn, {Anne C.}",

note = "Funding Information: The authors are grateful to Micbelle Beer and Kenneth Kobagen for their technical assistance. A.C~. was supported by NINCDS Teacher-Investigator Award NS00468. The continued support of the Genetics Center of Case Western Reserve University is also gratefully acknowledged. Additional support for this work was provided by NSF PCM 81-OO308 (to A.C~.). E~. received support from the Department of Psychobiology at Oberlin College, and J. D. S. was the recipient of a Strnad Fellowship from University School, Hunting Valley, Ohio.",

year = "1986",

month = apr,

day = "14",

doi = "10.1016/0024-3205(86)90469-8",

language = "English (US)",

volume = "38",

pages = "1369--1373",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "15",

}

TY - JOUR

T1 - Cations decrease specific [3H]-spiroperidol binding in human prefrontal cortex

AU - Huang, Emina H.

AU - Pickett, John M.

AU - Siegal, Joel D.

AU - Andorn, Anne C.

N1 - Funding Information: The authors are grateful to Micbelle Beer and Kenneth Kobagen for their technical assistance. A.C~. was supported by NINCDS Teacher-Investigator Award NS00468. The continued support of the Genetics Center of Case Western Reserve University is also gratefully acknowledged. Additional support for this work was provided by NSF PCM 81-OO308 (to A.C~.). E~. received support from the Department of Psychobiology at Oberlin College, and J. D. S. was the recipient of a Strnad Fellowship from University School, Hunting Valley, Ohio.

PY - 1986/4/14

Y1 - 1986/4/14

N2 - Ligand binding at many physiologically relevant receptors is regulated by divalent cations. To determine whether [3H]-spiroperidol binding sites in prefrontal cortex might be physiologically relevant receptors, we examined the effect of ions on the binding of this ligand in postmortem human prefrontal cortex. Our results indicate that several cations decreased [3H]-spiroperidol binding in a dose-dependent fashion. Of these, Cd++ and Zn++ were the most able to decrease [3H]-spiroperidol binding with IC50 of 5.5.5 +/- 2.4 × 10-6M and 5.6 +/- 1.1 × 10-5M respectively. These findings indicate that [3H]-spiroperidol may bind at physiologically relevant receptors in human prefrontal cortex.

AB - Ligand binding at many physiologically relevant receptors is regulated by divalent cations. To determine whether [3H]-spiroperidol binding sites in prefrontal cortex might be physiologically relevant receptors, we examined the effect of ions on the binding of this ligand in postmortem human prefrontal cortex. Our results indicate that several cations decreased [3H]-spiroperidol binding in a dose-dependent fashion. Of these, Cd++ and Zn++ were the most able to decrease [3H]-spiroperidol binding with IC50 of 5.5.5 +/- 2.4 × 10-6M and 5.6 +/- 1.1 × 10-5M respectively. These findings indicate that [3H]-spiroperidol may bind at physiologically relevant receptors in human prefrontal cortex.

UR - http://www.scopus.com/inward/record.url?scp=0022538745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022538745&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(86)90469-8

DO - 10.1016/0024-3205(86)90469-8

M3 - Article

C2 - 3959758

AN - SCOPUS:0022538745

SN - 0024-3205

VL - 38

SP - 1369

EP - 1373

JO - Life Sciences

JF - Life Sciences

IS - 15

ER -

Cations decrease specific [³H]-spiroperidol binding in human prefrontal cortex

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this