Abstract
The addition of lanthanide ions to catalytically inactive apo alkaline phosphatase results in less than 5 percent of the native catalytic activity. Attempts to distinquish whether this catalytic activity results for a lanthanide ion-alkaline phosphatase complex or contamination with zinc ion have thus for been unsuccessful. In an attempt to examine the role of the metal ions in the enzymic reaction catalyzed by alkaline phosphatase attempts have been made to demonstrate whether substrates enter the primary coordination sphere of the bound metal. Addition of glucose-6-phosphate, phosphoenolypyruvate or inorganic phosphate result in a quenching of the luminescence of the terbium-alkaline phosphatase complex. This decrease in luminescence is a result of a change in the environment around the bound terbium ion either by direct binding of substrate to the terbium or a substrate induced conformational change.
| Original language | English (US) |
|---|---|
| Title of host publication | Unknown Host Publication Title |
| Place of Publication | Oak Ridge, Tenn |
| Publisher | AEC |
| Pages | 204-212 |
| Number of pages | 9 |
| State | Published - 1800 |
| Event | Rare Earth Res Conf, 11th, Proc - Traverse City, MI, USA Duration: Oct 7 1974 → Oct 10 1974 |
Other
| Other | Rare Earth Res Conf, 11th, Proc |
|---|---|
| City | Traverse City, MI, USA |
| Period | 10/7/74 → 10/10/74 |
ASJC Scopus subject areas
- General Engineering