Cardioprotective effects of 70-kDa heat shock protein in transgenic mice

Nina B. Radford; Maggy Fina; Ivor J. Benjamin; Randall W. Moreadith; Kathy H. Graves; Piyu Zhao; Sandhya Gavva; Andrea Wiethoff; A. Dean Sherry; Craig R. Malloy; R. Sanders Williams

doi:10.1073/pnas.93.6.2339

Cardioprotective effects of 70-kDa heat shock protein in transgenic mice

Nina B. Radford, Maggy Fina, Ivor J. Benjamin, Randall W. Moreadith, Kathy H. Graves, Piyu Zhao, Sandhya Gavva, Andrea Wiethoff, A. Dean Sherry, Craig R. Malloy, R. Sanders Williams

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Abstract

Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70- kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.

Original language	English (US)
Pages (from-to)	2339-2342
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	93
Issue number	6
DOIs	https://doi.org/10.1073/pnas.93.6.2339
State	Published - Mar 19 1996

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Radford, N. B., Fina, M., Benjamin, I. J., Moreadith, R. W., Graves, K. H., Zhao, P., Gavva, S., Wiethoff, A., Sherry, A. D., Malloy, C. R., & Williams, R. S. (1996). Cardioprotective effects of 70-kDa heat shock protein in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America, 93(6), 2339-2342. https://doi.org/10.1073/pnas.93.6.2339

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abstract = "Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70- kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.",

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AU - Radford, Nina B.

AU - Fina, Maggy

AU - Benjamin, Ivor J.

AU - Moreadith, Randall W.

AU - Graves, Kathy H.

AU - Zhao, Piyu

AU - Gavva, Sandhya

AU - Wiethoff, Andrea

AU - Sherry, A. Dean

AU - Malloy, Craig R.

AU - Williams, R. Sanders

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AB - Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70- kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.

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Cardioprotective effects of 70-kDa heat shock protein in transgenic mice

Abstract

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