C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism

F. Mark H Jeffrey; Arun Rajagopal; Craig R. Malloy; A. Dear Sherry

doi:10.1016/0968-0004(91)90004-F

C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism

F. Mark H Jeffrey, Arun Rajagopal, Craig R. Malloy, A. Dear Sherry

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Abstract

¹³C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual ¹³C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.

Original language	English (US)
Pages (from-to)	5-10
Number of pages	6
Journal	Trends in biochemical sciences
Volume	16
Issue number	C
DOIs	https://doi.org/10.1016/0968-0004(91)90004-F
State	Published - 1991

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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title = "C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism",

abstract = "13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.",

author = "Jeffrey, {F. Mark H} and Arun Rajagopal and Malloy, {Craig R.} and Sherry, {A. Dear}",

note = "Funding Information: The authors appreciate assistance with heart perfusions by D. Drew and H. Stere, and secretarial assistance from D. Shuttlesworth. This work was supported by a Research Associate Award and Merit Review from the Veterans Administration, NIH HL34557, and the Meadows Foundation.",

year = "1991",

doi = "10.1016/0968-0004(91)90004-F",

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T2 - a simple yet comprehensive method for analysis of intermediary metabolism

AU - Jeffrey, F. Mark H

AU - Rajagopal, Arun

AU - Malloy, Craig R.

AU - Sherry, A. Dear

N1 - Funding Information: The authors appreciate assistance with heart perfusions by D. Drew and H. Stere, and secretarial assistance from D. Shuttlesworth. This work was supported by a Research Associate Award and Merit Review from the Veterans Administration, NIH HL34557, and the Meadows Foundation.

PY - 1991

Y1 - 1991

N2 - 13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.

AB - 13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.

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C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism

Abstract

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