13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.
|Original language||English (US)|
|Number of pages||6|
|Journal||Trends in biochemical sciences|
|State||Published - 1991|
ASJC Scopus subject areas
- Molecular Biology