c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia

Marjatta Son; Asha R. Shahed; Paul M. Werchan; John C. Lee

doi:10.1016/0304-3940(95)12114-J

c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia

Marjatta Son, Asha R. Shahed, Paul M. Werchan, John C. Lee

Neurology & Neurotherapeutics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Previous studies have shown that brief exposures of rodents to high gravitation forces (+G_Z) in a specifically designed centrifuge cause global cerebral ischemia. In the present study, the effect of +G_Z exposure to +22.5G_Z for 15 to 60 s on c-fos and HSP70 gene expression was examined. Northern and RT-PCR analyses of total RNA isolated from brains of rats at different post-exposure times revealed a significant, time-dependent increase in the c-fos mRNA level which returned to near normal by 180 min. The HSP70 mRNA level was increased two-fold at 30 min post exposure, and remained elevated until 180 min. The transient stimulation of c-fos and HSP70 gene expression should serve as useful biomarkers for hypergravic stress on the brain. The present results should aid in design of future experiments in our understanding of the pathophysiology of the high +G_Z challenges.

Original language	English (US)
Pages (from-to)	81-84
Number of pages	4
Journal	Neuroscience letters
Volume	200
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(95)12114-J
State	Published - Nov 17 1995

Keywords

Cerebral ischemia
Gene expression
Gravitation force
HSP70
Loss of consciousness
c-fos

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(95)12114-J

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title = "c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia",

abstract = "Previous studies have shown that brief exposures of rodents to high gravitation forces (+GZ) in a specifically designed centrifuge cause global cerebral ischemia. In the present study, the effect of +GZ exposure to +22.5GZ for 15 to 60 s on c-fos and HSP70 gene expression was examined. Northern and RT-PCR analyses of total RNA isolated from brains of rats at different post-exposure times revealed a significant, time-dependent increase in the c-fos mRNA level which returned to near normal by 180 min. The HSP70 mRNA level was increased two-fold at 30 min post exposure, and remained elevated until 180 min. The transient stimulation of c-fos and HSP70 gene expression should serve as useful biomarkers for hypergravic stress on the brain. The present results should aid in design of future experiments in our understanding of the pathophysiology of the high +GZ challenges.",

keywords = "Cerebral ischemia, Gene expression, Gravitation force, HSP70, Loss of consciousness, c-fos",

author = "Marjatta Son and Shahed, {Asha R.} and Werchan, {Paul M.} and Lee, {John C.}",

note = "Funding Information: Research sponsored by the Air Force Office of Scientific Research, Air Force Systems Command, USAF, under grant number AFOSR F49620-93-1-0029.",

year = "1995",

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doi = "10.1016/0304-3940(95)12114-J",

language = "English (US)",

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pages = "81--84",

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T1 - c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia

AU - Son, Marjatta

AU - Shahed, Asha R.

AU - Werchan, Paul M.

AU - Lee, John C.

N1 - Funding Information: Research sponsored by the Air Force Office of Scientific Research, Air Force Systems Command, USAF, under grant number AFOSR F49620-93-1-0029.

PY - 1995/11/17

Y1 - 1995/11/17

N2 - Previous studies have shown that brief exposures of rodents to high gravitation forces (+GZ) in a specifically designed centrifuge cause global cerebral ischemia. In the present study, the effect of +GZ exposure to +22.5GZ for 15 to 60 s on c-fos and HSP70 gene expression was examined. Northern and RT-PCR analyses of total RNA isolated from brains of rats at different post-exposure times revealed a significant, time-dependent increase in the c-fos mRNA level which returned to near normal by 180 min. The HSP70 mRNA level was increased two-fold at 30 min post exposure, and remained elevated until 180 min. The transient stimulation of c-fos and HSP70 gene expression should serve as useful biomarkers for hypergravic stress on the brain. The present results should aid in design of future experiments in our understanding of the pathophysiology of the high +GZ challenges.

AB - Previous studies have shown that brief exposures of rodents to high gravitation forces (+GZ) in a specifically designed centrifuge cause global cerebral ischemia. In the present study, the effect of +GZ exposure to +22.5GZ for 15 to 60 s on c-fos and HSP70 gene expression was examined. Northern and RT-PCR analyses of total RNA isolated from brains of rats at different post-exposure times revealed a significant, time-dependent increase in the c-fos mRNA level which returned to near normal by 180 min. The HSP70 mRNA level was increased two-fold at 30 min post exposure, and remained elevated until 180 min. The transient stimulation of c-fos and HSP70 gene expression should serve as useful biomarkers for hypergravic stress on the brain. The present results should aid in design of future experiments in our understanding of the pathophysiology of the high +GZ challenges.

KW - Cerebral ischemia

KW - Gene expression

KW - Gravitation force

KW - HSP70

KW - Loss of consciousness

KW - c-fos

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JO - Neuroscience letters

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c-fos and HSP70 gene expression in rat brains in high gravitation-induced cerebral ischemia

Abstract

Keywords

ASJC Scopus subject areas

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