TY - JOUR
T1 - Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A
AU - Kumagai, H.
AU - Tomoda, H.
AU - Omura, S.
PY - 1992
Y1 - 1992
N2 - The biosynthesis of antibiotic 1233A (F-244) was studied by feeding 13C-labeled precursors to the producing organism, Scopulariopsis sp. F-244. 13C NMR spectroscopy established that 1233A is derived from 4 methionines and 7 acetates. Seven acetates are condensed to form a hexaketide and 4 methyl residues from methionine are introduced into the main skeleton. The β-lactone is derived from the α-carboxylic acid of the hexaketide. Since methionine was efficiently incorporated into 1233A, radiolabeled 1233A was prepared biosynthetically by feeding [14C]methionine to the producer. As a result, [I4C]1233A was obtained with high specific radioactivity (27.2μCi/μmol).
AB - The biosynthesis of antibiotic 1233A (F-244) was studied by feeding 13C-labeled precursors to the producing organism, Scopulariopsis sp. F-244. 13C NMR spectroscopy established that 1233A is derived from 4 methionines and 7 acetates. Seven acetates are condensed to form a hexaketide and 4 methyl residues from methionine are introduced into the main skeleton. The β-lactone is derived from the α-carboxylic acid of the hexaketide. Since methionine was efficiently incorporated into 1233A, radiolabeled 1233A was prepared biosynthetically by feeding [14C]methionine to the producer. As a result, [I4C]1233A was obtained with high specific radioactivity (27.2μCi/μmol).
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U2 - 10.7164/antibiotics.45.563
DO - 10.7164/antibiotics.45.563
M3 - Article
C2 - 1350579
AN - SCOPUS:0026506664
SN - 0021-8820
VL - 45
SP - 563
EP - 567
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 4
ER -