@article{d0a65735dbb749b29a170d63951e4141,
title = "Bax is necessary for PGC1α pro-apoptotic effect in colorectal cancer cells",
abstract = "We have recently shown that the transcriptional coactivator PGC1α, a master regulator of mitochondrial biogenesis and function, is involved in the control of the intestinal epithelium cell fate. Furthermore, PGC1α protects against colon cancer formation by promoting ROS accumulation and, consequently, mitochondria-mediated apoptosis. Here we provide an additional mechanistic insight into the tumor suppressor activity of PGC1α showing that its pro-apoptotic effect is mediated by Bax. In fact, PGC1α overexpression in HCT116 Bax-/- colorectal cancer cells stimulates mitochondrial production and activity, but it fails to induce cell death as well as to oppose tumor growth in the xenograft model. The lack of ROS accumulation in the Bax-/- cells strengthens our view that the PGC1α-induced oxidative burst represents one of the main apoptosis-driving factors in colorectal cancer cells.",
keywords = "Apoptosis, Intestine, Mitochondria, Nuclear receptors, Reactive oxygen species (ROS)",
author = "Ilenia D'Errico and {Lo Sasso}, Giuseppe and Lorena Salvatore and Stefania Murzilli and Nicola Martelli and Maricarmen Cristofaro and Dominga Latorre and Gaetano Villani and Antonio Moschetta",
note = "Funding Information: ROS staining was performed as previously reported in ref-We are indebted to C. Simone and B. Vogelstein for the erence 6. Briefly, cryostat section 10 μm were incubated in a HCT116 Bax-/- cell line. We thank A. D{\textquoteright}Orazio and R. Le medium containing 12.5 mM DAB, 5 mM MnCl2and 40 mM Donne for their precious help during the study. A. Moschetta CoCl2 dissolved in 10%, in 100 mM Tris-maleate buffer (pH is funded by the Italian Association for Cancer Research 8.0) at 37°C. After 30 min incubation the section were rinsed in (AIRC, IG 10416), Italian Ministry of University (FIRB hot distilled water (60°C) to stop the reaction and to remove the IDEAS RBID08C9N7), Italian Ministry of Health (Young viscous incubation medium. Data obtained on ROS and COX Researchers Grant GR-2008-1143546), European Community{\textquoteright}s has been quantified through the image processing and analysis Seventh Framework Programme FP7/2007–2013 under Grant tools from the ImageJ software (v.1.43, Ferreira and Rasband, Agreement No. 202272 (LipidomicNet), Telethon (GPP08259), http://rsbweb.nih.gov/ij/). Cariplo (Milan), Natural Pharma International and University RNA extraction. Total RNA was isolated by TRIzol reagent of Bari (IDEA GRBA0802SJ-2008, ORBA10CDZI-2010). G. (Invitrogen) following the manufacture{\textquoteright}s instruction. To avoid Villani is funded by PRIN 2006 n. 2006069034_004 of the possible DNA contaminations, RNA was treated with DNAase-1 Italian Ministry of the University. S. Murzilli was supported by (Ambion, Foster City, CA). RNA purity was checked by spec-a fellowship from CariSPAQ (L{\textquoteright}Aquila). I. D{\textquoteright}Errico and G. Lo trophotometer and RNA integrity by examination on agarose gel Sasso were fellows of AIRC.",
year = "2011",
month = sep,
day = "1",
doi = "10.4161/cc.10.17.16791",
language = "English (US)",
volume = "10",
pages = "2937--2945",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "17",
}