TY - JOUR
T1 - BAC/YAC contigs from the H2-M region of mouse Chr 17 define gene order as Znf173-Tctex5-Mog-D17Tu42-M3-M2
AU - Yoshino, Masayasu
AU - Xiao, Hong
AU - Jones, Elsy P.
AU - Lindahl, Kirsten Fischer
N1 - Funding Information:
AcknowledgmentsmM. Yoshino was supported by the Japan Society for Promotion of Science (JSPS) as a JSPS Research Fellow in 1995 and as a JSPS Postdoctoral Fellow Abroad in 1996 and 1997. We greatly appreciate Derek Byers for his help with equipment maintenance and chromosome preparation. We thank Dr. Roger Schultz for access to FISH equipment, Claire Amadou for the Tctex5 cDNA sequence from H. Uehara, Attila Kumánovics for discussions on the H2-M genes, Irene Carlo for plasmid clones, and Natsuki Yoshino for reading X-ray films of sequencing gels. This work was supported by the NIH AI37818 grant.
PY - 1998
Y1 - 1998
N2 - A yeast artificial chromosome (YAC) contig from the C57BL/6 (H2b) mouse was created from the major histocompatibility complex (Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous > 1.5-Mb YAC contigs into a single contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 (H2(bc)) mouse, spanning approximately 600 kilobases, was also built from Znf173 (Afp, a gene for acid finger protein), through Tctex5 (t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs, which define the marker order as Cen-Znf173-Tctex5-Mog-D17Tu42-D17Mit232-H2-M3-D17Leh525-H2-M2-Tel. The gene order of Znf173-Tctex5-Mog-D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A.
AB - A yeast artificial chromosome (YAC) contig from the C57BL/6 (H2b) mouse was created from the major histocompatibility complex (Mhc, H2 in mouse) class Ib subregion, H2-M. It spans approximately 1.2 megabase (Mb) pairs and unites the previous > 1.5-Mb YAC contigs into a single contig, which includes 21 Mhc class I genes distal to H2-T1. A bacterial artificial chromosome (BAC) contig from the 129 (H2(bc)) mouse, spanning approximately 600 kilobases, was also built from Znf173 (Afp, a gene for acid finger protein), through Tctex5 (t-complex testis expressed-5) and Mog (myelin oligodendrocyte glycoprotein), to H2-M2. Twenty-four sequence-tagged site (STS) markers were newly developed, and 35 markers were mapped in the YAC/BAC contigs, which define the marker order as Cen-Znf173-Tctex5-Mog-D17Tu42-D17Mit232-H2-M3-D17Leh525-H2-M2-Tel. The gene order of Znf173-Tctex5-Mog-D17Tu42 is conserved between mouse and human, showing that the middle H2-M region corresponds to the subregion of the human Mhc surrounding HLA-A.
KW - Evolution
KW - HLA
KW - Major histocompatibility complex (MHC)
KW - Physical mapping
KW - Sequence tagged sites (STS)
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U2 - 10.1007/s002510050372
DO - 10.1007/s002510050372
M3 - Article
C2 - 9510555
AN - SCOPUS:0031939236
SN - 0093-7711
VL - 47
SP - 371
EP - 380
JO - Immunogenetics
JF - Immunogenetics
IS - 5
ER -