TY - JOUR
T1 - Avian and murine lr8b and human apolipoprotein E receptor 2
T2 - Differentially spliced products from corresponding genes
AU - Brandes, Christian
AU - Novak, Sabine
AU - Stockinger, Walter
AU - Herz, Joachim
AU - Schneider, Wolfgang J.
AU - Nimpf, Johannes
N1 - Funding Information:
We appreciate the expert technical assistance by Harald Rumpler. This work was supported by grants from the Austrian Science Foundation S-07105 (to J.N.) and S-07108 (to W.J.S.). We thank Dr. Franz Wohlrab and Dr. Reinhold Hofbauer for critically reading the manuscript.
PY - 1997/6/1
Y1 - 1997/6/1
N2 - Apolipoprotein E-mediated lipid metabolism in the central nervous system plays an important role in cholesterol and phospholipid homeostasis of this organ, which is separated from the circulation by the blood-brain barrier. Moreover, in late-onset familial Alzheimer disease the frequency of the apolipoprotein E4 allele is significantly increased and the apoprotein is localized to extracellular plaques, one of the histological hallmarks of this disease. Recently, two distinct novel members of the low-density lipoprotein (LDL) receptor family, with the potential to bind apolipoprotein E and preferentially expressed in brain, have been characterized from human (D. Kim et al., 1996, J. Biol. Chem 271: 8373-8380) and chicken and mouse (S. Novak, et al., 1996, J. Biol. Chem. 271: 11732-11736). The human receptor, termed 'apolipoprotein E receptor 2,' is a seven ligand-binding repeat receptor harboring a unique insertion in the cytoplasmic domain of the protein. The novel receptor characterized in chicken and mouse was found to have eight binding repeats without such a cytoplasmic insertion. Despite the overall identity of more than 73%, based upon their structural differences (seven versus eight ligand-binding repeats) these receptors have been considered independent entities. However, here we demonstrate that both receptors in fact are encoded by corresponding genes and that differential splicing gives rise to structurally and possibly functionally distinct variants of this brain-specific member of the LDL receptor family.
AB - Apolipoprotein E-mediated lipid metabolism in the central nervous system plays an important role in cholesterol and phospholipid homeostasis of this organ, which is separated from the circulation by the blood-brain barrier. Moreover, in late-onset familial Alzheimer disease the frequency of the apolipoprotein E4 allele is significantly increased and the apoprotein is localized to extracellular plaques, one of the histological hallmarks of this disease. Recently, two distinct novel members of the low-density lipoprotein (LDL) receptor family, with the potential to bind apolipoprotein E and preferentially expressed in brain, have been characterized from human (D. Kim et al., 1996, J. Biol. Chem 271: 8373-8380) and chicken and mouse (S. Novak, et al., 1996, J. Biol. Chem. 271: 11732-11736). The human receptor, termed 'apolipoprotein E receptor 2,' is a seven ligand-binding repeat receptor harboring a unique insertion in the cytoplasmic domain of the protein. The novel receptor characterized in chicken and mouse was found to have eight binding repeats without such a cytoplasmic insertion. Despite the overall identity of more than 73%, based upon their structural differences (seven versus eight ligand-binding repeats) these receptors have been considered independent entities. However, here we demonstrate that both receptors in fact are encoded by corresponding genes and that differential splicing gives rise to structurally and possibly functionally distinct variants of this brain-specific member of the LDL receptor family.
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U2 - 10.1006/geno.1997.4702
DO - 10.1006/geno.1997.4702
M3 - Article
C2 - 9192837
AN - SCOPUS:0031172070
SN - 0888-7543
VL - 42
SP - 185
EP - 191
JO - Genomics
JF - Genomics
IS - 2
ER -