Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

Chang Seok Oh; Sang Jun Lee; Jun Bum Park; Soong Deok Lee; Seung Bum Seo; Hye Yeon Kim; Jaehyup Kim; Yi Suk Kim; Dong Hoon Shin

doi:10.1111/j.1556-4029.2011.02044.x

Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

Chang Seok Oh, Sang Jun Lee, Jun Bum Park, Soong Deok Lee, Seung Bum Seo, Hye Yeon Kim, Jaehyup Kim, Yi Suk Kim, Dong Hoon Shin

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR ^® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR ^® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.

Original language	English (US)
Pages (from-to)	820-825
Number of pages	6
Journal	Journal of Forensic Sciences
Volume	57
Issue number	3
DOIs	https://doi.org/10.1111/j.1556-4029.2011.02044.x
State	Published - May 2012
Externally published	Yes

Keywords

AmpFℓSTR Identifiler™ kit
AmpFℓSTR MiniFiler™ kit
Ancient DNA
Autosomal short tandem repeat genotyping
Forensic science
Korea
Medieval human sample

ASJC Scopus subject areas

Pathology and Forensic Medicine
Genetics

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10.1111/j.1556-4029.2011.02044.x

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title = "Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits",

abstract = "Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR {\textregistered} MiniFiler{\texttrademark} kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR {\textregistered} MiniFiler{\texttrademark} and Identifiler{\texttrademark} kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler{\texttrademark} kit, 75% of the same loci profiles were determined by MiniFiler{\texttrademark} kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler{\texttrademark} kit could be a useful complement to conventional STR kit analysis of ancient samples.",

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author = "Oh, {Chang Seok} and Lee, {Sang Jun} and Park, {Jun Bum} and Lee, {Soong Deok} and Seo, {Seung Bum} and Kim, {Hye Yeon} and Jaehyup Kim and Kim, {Yi Suk} and Shin, {Dong Hoon}",

year = "2012",

month = may,

doi = "10.1111/j.1556-4029.2011.02044.x",

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AU - Oh, Chang Seok

AU - Lee, Sang Jun

AU - Park, Jun Bum

AU - Lee, Soong Deok

AU - Seo, Seung Bum

AU - Kim, Hye Yeon

AU - Kim, Jaehyup

AU - Kim, Yi Suk

AU - Shin, Dong Hoon

PY - 2012/5

Y1 - 2012/5

N2 - Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR ® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR ® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.

AB - Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFℓSTR ® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n=8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFℓSTR ® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.

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KW - AmpFℓSTR MiniFiler™ kit

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Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

Abstract

Keywords

ASJC Scopus subject areas

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