Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

Joseph J. Yohn; Christopher Smith; Troy Stevens; Joseph G. Morelli; Libby R. Shurnas; Sandra J. Walchak; Talia A. Hoffman; Kim K. Kelley; Antonio Escobedo-Morse; Masashi Yanagisawa; Madeleine A. Kane; Martin R. Zamora

doi:10.1016/0167-4889(94)90282-8

Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

Joseph J. Yohn, Christopher Smith, Troy Stevens, Joseph G. Morelli, Libby R. Shurnas, Sandra J. Walchak, Talia A. Hoffman, Kim K. Kelley, Antonio Escobedo-Morse, Masashi Yanagisawa, Madeleine A. Kane, Martin R. Zamora

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24 Scopus citations

Abstract

We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ET_B receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

Original language	English (US)
Pages (from-to)	454-458
Number of pages	5
Journal	BBA - Molecular Cell Research
Volume	1224
Issue number	3
DOIs	https://doi.org/10.1016/0167-4889(94)90282-8
State	Published - Dec 30 1994

Keywords

(Human)
Calcium
Endothelin-1
Keratinocyte
Receptor

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/0167-4889(94)90282-8

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Yohn, J. J., Smith, C., Stevens, T., Morelli, J. G., Shurnas, L. R., Walchak, S. J., Hoffman, T. A., Kelley, K. K., Escobedo-Morse, A., Yanagisawa, M., Kane, M. A., & Zamora, M. R. (1994). Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor. BBA - Molecular Cell Research, 1224(3), 454-458. https://doi.org/10.1016/0167-4889(94)90282-8

Yohn, JJ, Smith, C, Stevens, T, Morelli, JG, Shurnas, LR, Walchak, SJ, Hoffman, TA, Kelley, KK, Escobedo-Morse, A, Yanagisawa, M, Kane, MA & Zamora, MR 1994, 'Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor', BBA - Molecular Cell Research, vol. 1224, no. 3, pp. 454-458. https://doi.org/10.1016/0167-4889(94)90282-8

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title = "Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor",

abstract = "We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.",

keywords = "(Human), Calcium, Endothelin-1, Keratinocyte, Receptor",

author = "Yohn, {Joseph J.} and Christopher Smith and Troy Stevens and Morelli, {Joseph G.} and Shurnas, {Libby R.} and Walchak, {Sandra J.} and Hoffman, {Talia A.} and Kelley, {Kim K.} and Antonio Escobedo-Morse and Masashi Yanagisawa and Kane, {Madeleine A.} and Zamora, {Martin R.}",

note = "Funding Information: This work was supportedb y: NIH grant KO8 ARO1868-02( J.J.Y.), AmericanC ancerS ocietyR e-search Grant CD513 (M.A.K.), The Robert Wood Johnson Foundationa nd the SclerodermRa esearch Foundation(M .R.Z.).",

year = "1994",

month = dec,

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doi = "10.1016/0167-4889(94)90282-8",

language = "English (US)",

volume = "1224",

pages = "454--458",

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T1 - Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

AU - Yohn, Joseph J.

AU - Smith, Christopher

AU - Stevens, Troy

AU - Morelli, Joseph G.

AU - Shurnas, Libby R.

AU - Walchak, Sandra J.

AU - Hoffman, Talia A.

AU - Kelley, Kim K.

AU - Escobedo-Morse, Antonio

AU - Yanagisawa, Masashi

AU - Kane, Madeleine A.

AU - Zamora, Martin R.

N1 - Funding Information: This work was supportedb y: NIH grant KO8 ARO1868-02( J.J.Y.), AmericanC ancerS ocietyR e-search Grant CD513 (M.A.K.), The Robert Wood Johnson Foundationa nd the SclerodermRa esearch Foundation(M .R.Z.).

PY - 1994/12/30

Y1 - 1994/12/30

N2 - We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

AB - We investigated endothelin-1 (ET-1) receptor expression on normal human keratinocytes (HK). We show that HK express the ETB receptor isoform and respond to ET-1 with a 2.7-fold increase in intracellular free calcium. HK did not respond to ET-1 with increased proliferation; however, 30 nM ET-1 caused a 51.7% decrease in ET-1 accumulation in HK-conditioned medium. We propose that HK ET-1 receptors function in autocrine regulation of ET-1 secretion.

KW - (Human)

KW - Calcium

KW - Endothelin-1

KW - Keratinocyte

KW - Receptor

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AN - SCOPUS:0028574308

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JF - BBA - Molecular Cell Research

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Autoregulation of endothelin-1 secretion by cultured human keratinocytes via the endothelin B receptor

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