@inbook{7699e12cd4594b738fac62a7e1afb1f5,
title = "Assessment of Posttranslational Modifications of ATG proteins",
abstract = "Posttranslational modifications (PTMs) increase the functional diversity of proteins and play a key role in many cellular processes. Macroautophagy (hereafter simply referred to as autophagy) is an evolutionarily conserved, lysosome-dependent degradation pathway. This process is finely regulated by autophagy-related (ATG) genes widely conserved among eukaryotes from yeast to mammals. Various PTMs of ATG proteins such as phosphorylation, ubiquitination, and acetylation have been theorized to play a critical role in modulating autophagic processes and activity. In this chapter, we introduce several antibody-based tools (e.g., Western blot, Simple Western{\texttrademark}, immunofluorescence, and immunoprecipitation) that are widely used to assess the PTMs of ATG proteins in mammalian cells.",
keywords = "Autophagy, Autophagy-related protein, Immunofluorescence, Immunoprecipitation, Posttranslational modification, Simple Western, Western blot",
author = "Y. Xie and R. Kang and D. Tang",
note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
doi = "10.1016/bs.mie.2016.09.057",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "171--188",
booktitle = "Methods in Enzymology",
}