Assessment of Posttranslational Modifications of ATG proteins

Y. Xie, R. Kang, D. Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


Posttranslational modifications (PTMs) increase the functional diversity of proteins and play a key role in many cellular processes. Macroautophagy (hereafter simply referred to as autophagy) is an evolutionarily conserved, lysosome-dependent degradation pathway. This process is finely regulated by autophagy-related (ATG) genes widely conserved among eukaryotes from yeast to mammals. Various PTMs of ATG proteins such as phosphorylation, ubiquitination, and acetylation have been theorized to play a critical role in modulating autophagic processes and activity. In this chapter, we introduce several antibody-based tools (e.g., Western blot, Simple Western™, immunofluorescence, and immunoprecipitation) that are widely used to assess the PTMs of ATG proteins in mammalian cells.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Number of pages18
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Autophagy
  • Autophagy-related protein
  • Immunofluorescence
  • Immunoprecipitation
  • Posttranslational modification
  • Simple Western
  • Western blot

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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