@inproceedings{7ced1ed166a747308d7c2aa546cf5853,
title = "Assessing breast cancer response to bevacizumab using contrast-enhanced ultrasound: Initial results using a murine model",
abstract = "This paper describes preliminary results in a murine model devoted to monitoring breast cancer response to bevacizumab therapy using contrast-enhanced ultrasound (US). Twenty mice were implanted with 2LMP breast cancer cells. Three weeks thereafter, 10 mice were administered a 0.1 mg i.p. injection of bevacizumab whereas the remaining mice received saline injections. Using a Philips iU22 scanner equipped with an L9-3 transducer, baseline and follow-up imaging at day 6 was performed using contrast-enhanced US and flash-replenishment techniques. Contrast agent flow (i.e., time-intensity curves) and tumor reperfusion parameters were derived from US image sequences and a given region-of-interest (ROI). Experimental results showed reperfusion metrics were greater for tumors treated with bevacizumab compared to controls. Significant differences found in the AUC (p < 0.01) and Ipk (p < 0.01) timeintensity curve parameters maybe due to a reduction in intratumoral pressures and restored tumor flow. No significant differences were found between control and treated tumors for either the Tpk parameter (p = 0.20) or tumor size (p = 0.18). Histological results revealed increased microvessel density (MVD) for control tumors (p < 0.01) when compared to treated mice. No significant differences in tumor necrosis levels (p = 0.18) were found between control and therapy tumor groups. Overall, these preliminary experiments demonstrate that changes in tumor vascularity and perfusion kinetics in response to antiangiogenic therapy can be distinguished using contrast-enhanced US techniques.",
keywords = "Bevacizumab, Breast cancer, Contrast agent, Ultrasound",
author = "Kenneth Hoyt and Warram, {Jason M.} and Heidi Umphrey and Lin Belt and Sharon Samuel and Karri Folks and Cecil Stockard and Mark Lockhart and Robbin, {Michelle L.} and William Grizzle and Zinn, {Kurt R.}",
year = "2009",
doi = "10.1109/ULTSYM.2009.5441595",
language = "English (US)",
isbn = "9781424443895",
series = "Proceedings - IEEE Ultrasonics Symposium",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "259--262",
booktitle = "2009 IEEE International Ultrasonics Symposium and Short Courses, IUS 2009",
note = "2009 IEEE International Ultrasonics Symposium, IUS 2009 ; Conference date: 20-09-2009 Through 23-09-2009",
}