TY - JOUR
T1 - Aptasensor for Quantifying Pancreatic Polypeptide
AU - Ali, Ahmed S.M.
AU - El-Halawany, Medhat S.
AU - Ibrahim, Sherif Abdelaziz
AU - Plückthun, Olga
AU - Khalil, Ahmed S.G.
AU - Mayer, Günter
N1 - Funding Information:
This work was partially funded by Erasmus mobility program and funds from the German Research Council (DFG, SFB1089 and MA 3442/7-1 to G.M.). Notes The authors declare no competing financial interest.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/2/8
Y1 - 2019/2/8
N2 - Herein, a simple colorimetric aptasensor based on unmodified gold nanoparticles (AuNPs) has been developed for quantifying pancreatic polypeptide (PP), a peptide hormone associated with nonfunctional neuroendocrine pancreatic tumor (NF-PNET). First, several PP-specific aptamers have been screened by systematic evolution of ligands by exponential enrichment. The generated aptamers showed specificity and affinity higher than those reported so far. The sensing platform was constructed based on the optical properties of AuNPs as well as the adsorption of single-stranded DNA aptamers. The high sensitivity of the developed aptasensor was achieved by optimizing the sensing conditions, including salt tolerance, time of reaction, and optimal aptamer concentration. The developed aptasensor showed a wide linear dynamic range (1-60 nM) with a limit of detection of 521 pM. Furthermore, the performance of the aptasensor was evaluated for the detection of PP in human serum and showed recovery values ranging between 92.4 and 93.9%. Therefore, the presented colorimetric aptasensor has a great potential in the diagnosis of NF-pNETs.
AB - Herein, a simple colorimetric aptasensor based on unmodified gold nanoparticles (AuNPs) has been developed for quantifying pancreatic polypeptide (PP), a peptide hormone associated with nonfunctional neuroendocrine pancreatic tumor (NF-PNET). First, several PP-specific aptamers have been screened by systematic evolution of ligands by exponential enrichment. The generated aptamers showed specificity and affinity higher than those reported so far. The sensing platform was constructed based on the optical properties of AuNPs as well as the adsorption of single-stranded DNA aptamers. The high sensitivity of the developed aptasensor was achieved by optimizing the sensing conditions, including salt tolerance, time of reaction, and optimal aptamer concentration. The developed aptasensor showed a wide linear dynamic range (1-60 nM) with a limit of detection of 521 pM. Furthermore, the performance of the aptasensor was evaluated for the detection of PP in human serum and showed recovery values ranging between 92.4 and 93.9%. Therefore, the presented colorimetric aptasensor has a great potential in the diagnosis of NF-pNETs.
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U2 - 10.1021/acsomega.8b03131
DO - 10.1021/acsomega.8b03131
M3 - Article
AN - SCOPUS:85061334154
SN - 2470-1343
VL - 4
SP - 2948
EP - 2956
JO - ACS Omega
JF - ACS Omega
IS - 2
ER -