Anti receptor antibody. I. Isolation and characterization of the immunoglobulin receptor for phosphorylcholine

A phosphorylcholine binding protein in lysates from radioiodinated splenocytes of immunized mice was demonstrated. Immunoprecipitation with antireceptor antibody of lysates from splenocytes obtained from animals undergoing a primary response or from mice immunized 4 to 6 mth earlier with antigen demonstrated that this protein contained H and L chains. Therefore, an antigen specific receptor was isolated from the surface of spleen cells from both immunized and 'memory' animals. This receptor Ig comprises approximately 10% of total cell surface Ig.