TY - JOUR
T1 - Anti-prostate cancer activity of 8-hydroxyquinoline-2-carboxaldehyde-thiosemicarbazide copper complexes in vivo by bioluminescence imaging
AU - Xie, Fang
AU - Cai, Huawei
AU - Peng, Fangyu
N1 - Funding Information:
Acknowledgements The authors thank Haiyuan Zhang for synthesis of CuHQTS used for this study. This study was supported by a faculty research development fund awarded to F. P by Harold C. Simmons Comprehensive Cancer Center, UT Southwestern Medical Center for financial support. The study was also supported in part with infrastructure of the Southwestern Small Animal Imaging Resource provided by 1P30 CA142543 and P41-EB015908.
Funding Information:
The authors thank Haiyuan Zhang for synthesis of CuHQTS used for this study. This study was supported by a faculty research development fund awarded to F. P by Harold C. Simmons Comprehensive Cancer Center, UT Southwestern Medical Center for financial support. The study was also supported in part with infrastructure of the Southwestern Small Animal Imaging Resource provided by 1P30 CA142543 and P41-EB015908.
Publisher Copyright:
© 2018, SBIC.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Copper 8-hydroxyquinoline-2-carboxaldehyde-thiosemicarbazide complex (CuHQTS) is a copper complex with strong anticancer activity against cisplatin-resistant neuroblastoma and prostate cancer cells in vitro by cell proliferation assay or fluorescent microscopic imaging. This study aimed to evaluate anti-prostate cancer activity of CuHQTS in vivo by bioluminescence imaging (BLI) and tumor size measurement, using athymic nu/nu mice implanted with prostate cancer cells carrying luciferase reporter gene (Luc-PC3). Growth of Luc-PC3 cells (1 × 10 5 cells) implanted in athymic nu/nu mice treated with CuHQTS for 2 weeks was suppressed by measurement of luciferase signals (6.18 × 10 7 to 5.36 × 10 7 p/s/cm 2 /sr) with BLI, compared with luciferase signals of Luc-PC3 cells (4.66 × 10 7 to 1.51 × 10 8 p/s/cm 2 /sr, p < 0.05) in the mice treated with normal saline of placebo control. Moreover, the size of PC-3 xenograft tumor (126.5 ± 34.2 mm 3 ) in athymic nu/nu mice treated with CuHQTS was significantly smaller than the size of PC-3 xenograft tumor (218.6 ± 48.0 mm 3 , p < 0.05) in athymic nu/nu mice treated with normal saline of placebo control, suggesting in vivo tumor growth inhibition activity of CuHQTS on prostate cancer. The findings of this study support further investigation of CuHQTS as a promising new anticancer agent for the treatment of metastatic prostate cancer refractory to anticancer drugs currently available.
AB - Copper 8-hydroxyquinoline-2-carboxaldehyde-thiosemicarbazide complex (CuHQTS) is a copper complex with strong anticancer activity against cisplatin-resistant neuroblastoma and prostate cancer cells in vitro by cell proliferation assay or fluorescent microscopic imaging. This study aimed to evaluate anti-prostate cancer activity of CuHQTS in vivo by bioluminescence imaging (BLI) and tumor size measurement, using athymic nu/nu mice implanted with prostate cancer cells carrying luciferase reporter gene (Luc-PC3). Growth of Luc-PC3 cells (1 × 10 5 cells) implanted in athymic nu/nu mice treated with CuHQTS for 2 weeks was suppressed by measurement of luciferase signals (6.18 × 10 7 to 5.36 × 10 7 p/s/cm 2 /sr) with BLI, compared with luciferase signals of Luc-PC3 cells (4.66 × 10 7 to 1.51 × 10 8 p/s/cm 2 /sr, p < 0.05) in the mice treated with normal saline of placebo control. Moreover, the size of PC-3 xenograft tumor (126.5 ± 34.2 mm 3 ) in athymic nu/nu mice treated with CuHQTS was significantly smaller than the size of PC-3 xenograft tumor (218.6 ± 48.0 mm 3 , p < 0.05) in athymic nu/nu mice treated with normal saline of placebo control, suggesting in vivo tumor growth inhibition activity of CuHQTS on prostate cancer. The findings of this study support further investigation of CuHQTS as a promising new anticancer agent for the treatment of metastatic prostate cancer refractory to anticancer drugs currently available.
KW - Anticancer copper complex
KW - Bioluminescence Imaging
KW - CuHQTS
KW - Prostate cancer
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U2 - 10.1007/s00775-018-1596-y
DO - 10.1007/s00775-018-1596-y
M3 - Article
C2 - 30006867
AN - SCOPUS:85049897678
SN - 0949-8257
VL - 23
SP - 949
EP - 956
JO - Journal of Biological Inorganic Chemistry
JF - Journal of Biological Inorganic Chemistry
IS - 6
ER -