Annexin i targets S100C to early endosomes

Joachim Seemann; Klaus Weber; Volker Gerke

doi:10.1016/S0014-5793(97)00911-3

Annexin i targets S100C to early endosomes

Joachim Seemann, Klaus Weber, Volker Gerke

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61 Scopus citations

Abstract

Immunofluorescence and subcellular fractionation localize annexin I and the EF Band protein S100C to the same membranous structures which in part correspond to transferrin receptor-positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop-S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to co-localize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.

Original language	English (US)
Pages (from-to)	185-190
Number of pages	6
Journal	FEBS Letters
Volume	413
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)00911-3
State	Published - Aug 11 1997

Keywords

Annexin
Ca binding protein
EF hand
Endocytosis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00911-3

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title = "Annexin i targets S100C to early endosomes",

abstract = "Immunofluorescence and subcellular fractionation localize annexin I and the EF Band protein S100C to the same membranous structures which in part correspond to transferrin receptor-positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop-S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to co-localize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.",

keywords = "Annexin, Ca binding protein, EF hand, Endocytosis",

author = "Joachim Seemann and Klaus Weber and Volker Gerke",

note = "Funding Information: We thank Mary Osborn for helpful discussions. This work was supported in part by grants from the DFG and the HFSPO (to V.G.) and by a postdoctoral fellowship by the Max Planck society to J.S.",

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T1 - Annexin i targets S100C to early endosomes

AU - Seemann, Joachim

AU - Weber, Klaus

AU - Gerke, Volker

N1 - Funding Information: We thank Mary Osborn for helpful discussions. This work was supported in part by grants from the DFG and the HFSPO (to V.G.) and by a postdoctoral fellowship by the Max Planck society to J.S.

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Y1 - 1997/8/11

N2 - Immunofluorescence and subcellular fractionation localize annexin I and the EF Band protein S100C to the same membranous structures which in part correspond to transferrin receptor-positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop-S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to co-localize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.

AB - Immunofluorescence and subcellular fractionation localize annexin I and the EF Band protein S100C to the same membranous structures which in part correspond to transferrin receptor-positive endosomes. The association of S100C with endosomal membranes is strictly dependent on annexin I binding since a D91stop-S100C mutant protein, in which the residues essential for annexin I binding have been removed, fails to co-localize with membraneous structures. This indicates that annexin I and S100C form a complex in vivo and that the endosomal localization of this complex is mediated through an interaction of annexin I with the endosomal membrane.

KW - Annexin

KW - Ca binding protein

KW - EF hand

KW - Endocytosis

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Annexin i targets S100C to early endosomes

Abstract

Keywords

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