@article{31f76f5712234fb4bd1d23c57718321f,
title = "Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells",
abstract = "Successful ex vivo expansion of hematopoietic stem cells (HSCs) would greatly benefit the treatment of disease and the understanding of crucial questions of stem cell biology. Here we show, using microarray studies, that the HSC-supportive mouse fetal liver CD3+ cells specifically express the proteins angiopoietin-like 2 (Angptl2) and angiopoietin-like 3 (Angptl3). We observed a 24- or 30-fold net expansion of long-term HSCs by reconstitution analysis when we cultured highly enriched HSCs for 10 days in the presence of Angptl2 or Angptl3 together with saturating levels of other growth factors. The coiled-coil domain of Angptl2 was capable of stimulating expansion of HSCs. Furthermore, angiopoietin-like 5, angiopoietin-like 7 and microfibril-associated glycoprotein 4 also supported expansion of HSCs in culture.",
author = "Chengcheng Zhang and Megan Kaba and Guangtao Ge and Kathleen Xie and Wei Tong and Christopher Hug and Lodish, {Harvey F.}",
note = "Funding Information: We thank R&D Systems Inc. for providing purified bacterially expressed Angptl2, insect-expressed Angptl3 and bacterially expressed Angptl7, as well as monoclonal antibodies against human Angptl2. We are grateful to B. Zhou for help in protein expression, G. Paradis and M. Jennings in Massachusetts Institute of Technology flow cytometry core facility for cell sorting, and A. Babic for critically reading the manuscript. C.C.Z. is a Leukemia and Lymphoma Society Fellow. H.F.L. was supported by US National Institutes of Health grant R01 DK 067356 and from the Engineering Research Centers Program of the National Science Foundation under National Science Foundation Award Number EEC 9843342 through the Biotechnology Process Engineering Center at the Massachusetts Institute of Technology.",
year = "2006",
month = mar,
day = "14",
doi = "10.1038/nm1342",
language = "English (US)",
volume = "12",
pages = "240--245",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "2",
}