Abstract
We have examined the effect of androgen treatment on androgen receptor mRNA and protein expression in the LNCaP human prostate carcinoma cell line. Incubation with androgen caused a decrease in cellular androgen receptor mRNA content that was concentration and time dependent. Maximal suppression to approximately 35% of control level was observed after 49 h of exposure to androgen. By contrast, incubation of LNCaP cells with androgen resulted in a 2-fold increase in the cellular content of androgen receptor protein at 24 h. At 49 h androgen receptor protein increased 30% as assayed by immunoblots and 79% as assayed by ligand binding. These results suggest that ligand-induced changes in androgen receptor stability and/or the translational efficiency of androgen receptor mRNA account for the phenomenon of androgen receptor upregulation observed in cultured LNCaP cells. Furthermore, the suppression of androgen mRNA and protein that is caused by prolonged incubation with androgen is incomplete and is reversible upon removal of ligand.
Original language | English (US) |
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Pages (from-to) | 79-88 |
Number of pages | 10 |
Journal | Molecular and Cellular Endocrinology |
Volume | 76 |
Issue number | 1-3 |
DOIs | |
State | Published - Apr 1991 |
Keywords
- Androgen receptor
- Cell culture
- LNCaP
- Regulation
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology