TY - JOUR
T1 - Analysis of thymus-leukemia (TL) antigens with monoclonal antibodies
AU - McIntyre, K. R.
AU - Vitetta, E. S.
AU - Hammerling, U.
AU - Michaelson, J.
AU - Flaherty, L.
AU - Uhr, J. W.
PY - 1980/11/10
Y1 - 1980/11/10
N2 - Lysates of radioiodinated thymocytes have been sequentially immunoprecipitated with monoclonal antibodies specific for thymus-leukemia (TL) antigens. The TL antigenic specificities defined by the monoclonal antibodies appear not to correspond precisely to specificities previously defined with conventional alloantisera. Nonetheless, each of two monoclonal antibodies specific for a single TL determination was found to deplete lysates of B6.Tla(a) thymocytes of all molecules precipitable by an alloantiserum specific for the conventionally defined determinants TL.1,2,3,5, and 6. These results suggest that on B6.Tla(a) thymocytes, all the conventional TL antigenic specificities (TL.1,2,3,5,6) as well as the specificities defined by the monoclonal anti-TL antibodies (TL.m2,m3) are carried by a single molecular species.
AB - Lysates of radioiodinated thymocytes have been sequentially immunoprecipitated with monoclonal antibodies specific for thymus-leukemia (TL) antigens. The TL antigenic specificities defined by the monoclonal antibodies appear not to correspond precisely to specificities previously defined with conventional alloantisera. Nonetheless, each of two monoclonal antibodies specific for a single TL determination was found to deplete lysates of B6.Tla(a) thymocytes of all molecules precipitable by an alloantiserum specific for the conventionally defined determinants TL.1,2,3,5, and 6. These results suggest that on B6.Tla(a) thymocytes, all the conventional TL antigenic specificities (TL.1,2,3,5,6) as well as the specificities defined by the monoclonal anti-TL antibodies (TL.m2,m3) are carried by a single molecular species.
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M3 - Article
C2 - 7391571
AN - SCOPUS:0018942027
SN - 0022-1767
VL - 125
SP - 601
EP - 605
JO - Journal of Immunology
JF - Journal of Immunology
IS - 2
ER -