TY - JOUR
T1 - Analysis of radioactive and nonradioactive purine bases, nucleosides, and nucleotides by high-speed chromatography on a single column
AU - Bakay, Bohdan
AU - Nissinen, Erkki
AU - Sweetman, Lawrence
PY - 1978/5
Y1 - 1978/5
N2 - The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations of nonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system.
AB - The concentrations of radioactive and nonradioactive purine bases, purine nucleosides, purine mono-, di-, and trinucleotides in acid extracts of fibroblasts were determined by anion-exchange column chromatography. The concentrations of nonradioactive components were determined by computerized integration of the signal from a double-beam uv-detector. The radioactive metabolites were quantitated by high-efficiency, continuous liquid scintillation counting, employing a discrete sample transport system.
UR - http://www.scopus.com/inward/record.url?scp=0018071032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018071032&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(78)90319-6
DO - 10.1016/0003-2697(78)90319-6
M3 - Article
C2 - 655401
AN - SCOPUS:0018071032
SN - 0003-2697
VL - 86
SP - 65
EP - 77
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -