TY - JOUR
T1 - Analysis of endothelin related peptides in culture supernatant of porcine aortic endothelial cells
T2 - Evidence for biosynthetic pathway of endothelin-1
AU - Sawamura, Tatsuya
AU - Kimura, Sadao
AU - Shinmi, Osamu
AU - Sugita, Yoshiki
AU - Yanagisawa, Masashi
AU - Masaki, Tomoh
PY - 1989/8/15
Y1 - 1989/8/15
N2 - We investigated the molecular forms of endothelin (ET) related peptides in culture supernatant of porcine aortic endothelial cells by high performance liquid chromatography coupled with radioimmunoassays for ET related peptides. We isolated and sequenced a C-terminal peptide (big ET-1(22-39)) of big ET-1(1-39) and its N-terminal truncated form (big ET-1(23-39)) in addition to ET-1(1-21) and its oxidized form, [Met7(O)]ET-1(1-21). The total contents of the two C-terminal peptides of big ET-1(1-39) are approximately equal to those of ET-1(1-21) and its oxidized form on a molar basis in the culture supernatant. Furthermore, we isolated big ET-1(1-39) although its content is approximately 2% of that of ET-1(1-21). These results strongly suggest that ET-1(1-21) and big ET-1(22-39) are generated from big ET-1(1-39) by specific processing between Trp21-Val22.
AB - We investigated the molecular forms of endothelin (ET) related peptides in culture supernatant of porcine aortic endothelial cells by high performance liquid chromatography coupled with radioimmunoassays for ET related peptides. We isolated and sequenced a C-terminal peptide (big ET-1(22-39)) of big ET-1(1-39) and its N-terminal truncated form (big ET-1(23-39)) in addition to ET-1(1-21) and its oxidized form, [Met7(O)]ET-1(1-21). The total contents of the two C-terminal peptides of big ET-1(1-39) are approximately equal to those of ET-1(1-21) and its oxidized form on a molar basis in the culture supernatant. Furthermore, we isolated big ET-1(1-39) although its content is approximately 2% of that of ET-1(1-21). These results strongly suggest that ET-1(1-21) and big ET-1(22-39) are generated from big ET-1(1-39) by specific processing between Trp21-Val22.
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U2 - 10.1016/0006-291X(89)90813-9
DO - 10.1016/0006-291X(89)90813-9
M3 - Article
C2 - 2669747
AN - SCOPUS:0024341915
SN - 0006-291X
VL - 162
SP - 1287
EP - 1294
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -