Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

Hirohiko Ise; Seiji Takashima; Masato Nagaoka; Anwarul Ferdous; Toshihiro Akaike

doi:10.1023/A:1005412129500

Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

Hirohiko Ise, Seiji Takashima, Masato Nagaoka, Anwarul Ferdous, Toshihiro Akaike

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.

Original language	English (US)
Pages (from-to)	209-213
Number of pages	5
Journal	Biotechnology Letters
Volume	21
Issue number	3
DOIs	https://doi.org/10.1023/A:1005412129500
State	Published - 1999

Keywords

3D culture
Agarose gel
Aggregates
Hepatocytes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1023/A:1005412129500

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title = "Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel",

abstract = "Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.",

keywords = "3D culture, Agarose gel, Aggregates, Hepatocytes",

author = "Hirohiko Ise and Seiji Takashima and Masato Nagaoka and Anwarul Ferdous and Toshihiro Akaike",

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doi = "10.1023/A:1005412129500",

language = "English (US)",

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T1 - Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

AU - Ise, Hirohiko

AU - Takashima, Seiji

AU - Nagaoka, Masato

AU - Ferdous, Anwarul

AU - Akaike, Toshihiro

N1 - Funding Information: This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 1999

Y1 - 1999

N2 - Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.

AB - Three-dimensional (3D) and two-dimensional (2D) cultures of hepatocytes in various concentrations (0.3-0.7%) of agarose gel revealed that the hepatocytes under 3D cultures in 0.3% agarose gel possess long-term (> 3 weeks) viability, significant self-assembly to form tissue like aggregates, low lactate dehydrogenase release and high albumin synthesis. These were in contrast to 2D culture of hepatocytes. Our results suggest that the 3D culture of hepatocytes in agarose gel favors aggregate formation of functionally active cells and would be useful for liver transplantation as well as to analyze hepatocytes biology.

KW - 3D culture

KW - Agarose gel

KW - Aggregates

KW - Hepatocytes

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Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel

Abstract

Keywords

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