Analysis of Cell Proliferation, Senescence, and Cell Death in Zebrafish Embryos

Daniel Verduzco, James F. Amatruda

Research output: Book/ReportBook

44 Scopus citations

Abstract

Proper control of cell proliferation is critical for normal development, growth, differentiation, and tissue homeostasis. Dysregulation of cell division and cell death underlies almost all cancers, and contributes to the pathology of birth defects and degenerative diseases. The zebrafish has proved to be an excellent system for elucidating the roles of the cell cycle in normal development, and ways in which dysregulation of cell proliferation contributes to disease. This chapter describes the methods for studying the cell cycle in zebrafish embryos, including protocols to examine cell proliferation, DNA damage, senescence, and cell death.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages20
Volume101
DOIs
StatePublished - 2011

Publication series

NameMethods in Cell Biology
Volume101
ISSN (Print)0091679X

Keywords

  • Cell death
  • Cell proliferation
  • Differentiation
  • Dysregulation
  • Forward-genetic screen
  • Senescence

ASJC Scopus subject areas

  • Cell Biology

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