Adiponectin diminishes organ-specific microvascular endothelial cell activation associated with sepsis

Matijs Van Meurs, Pedro Castro, Nathan I. Shapiro, Shulin Lu, Midori Yano, Norikazu Maeda, Tohru Funahashi, Ichiro Shimomura, Jan G. Zijlstra, Grietje Molema, Samir M. Parikh, William C. Aird, Kiichiro Yano

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Experimental sepsis was induced in male C57BL/6j, adiponectin-deficient mice (ADPNKO), and wild-type littermates by i.p. injection of 16 mg/kg lipopolysaccharide or cecal ligation and puncture. Blood and tissue samples were harvested 24 h after model induction. Circulating adiponectin is reduced in mice with endotoxemic challenge and after cecal ligation and puncture compared with healthy control mice. Quantitative reverse transcriptase-polymerase chain reaction for adiponectin reveals a pattern of response that is both model-and organ-specific. When challenged with sepsis, adiponectin deficiency results in increased expression of endothelial adhesion and coagulation molecules in the lung, liver, and kidney as quantified by reverse transcriptase-polymerase chain reaction, increased macrophage and neutrophil infiltration by immunohistochemistry, and vascular leakage in the liver and kidney. Adiponectin-deficient mice have reduced survival following cecal ligation and puncture and increased blood levels of interleukin 6, soluble vascular endothelial growth factor receptor 1, and soluble endothelial adhesion molecules E-selectin and intercellular adhesion molecule 1. Finally, ADPNKO promoted end-organ injury in the liver and kidney, whereas the lungs were not affected. These data suggest a protective role of adiponectin in diminishing microvascular organ-specific endothelial cell activation during sepsis. ABBREVIATIONS: LPS-lipopolysaccharideCLP-cecal ligation and puncturesVEGFR1-soluble vascular endothelial growth factor receptor 1IL-6-interleukin 6ICAM-1-intercellular adhesion molecule 1VCAM-1-vascular cell adhesion molecule 1.

Original languageEnglish (US)
Pages (from-to)392-398
Number of pages7
Issue number4
StatePublished - Apr 2012
Externally publishedYes


  • Adiponectin
  • adhesion molecules
  • endothelium
  • multiple organ dysfunction
  • sepsis

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine


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