Acute Hypervitaminosis A in Guinea Pigs. I. Effects on Acid Hydrolases

G. WEISSMANN; J. W. UHR; L. THOMAS

doi:10.3181/00379727-112-28017

Acute Hypervitaminosis A in Guinea Pigs. I. Effects on Acid Hydrolases

G. WEISSMANN, J. W. UHR, L. THOMAS

Immunology

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13 Scopus citations

Abstract

Acute hypervitaminosis A was induced in guinea pigs by oral administration of Vit. A. This treatment caused much of the activity of acid phosphatase, beta-glucuronidase and cathepsin to become unsedimentable at 15,000 g in homogenates prepared in 0.25M sucrose. Lysosome-rich fractions of hypervitaminotic livers released beta-glucuronidase and cathepsin more readily into the incubation medium in vitro than did control fractions. These events were accompanied by increases of serum beta-glucuronidase, and are compatible with a direct effect of excess Vit. A on lysosomes.

Original language	English (US)
Pages (from-to)	284-287
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	112
Issue number	2
DOIs	https://doi.org/10.3181/00379727-112-28017
State	Published - Feb 1963

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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abstract = "Acute hypervitaminosis A was induced in guinea pigs by oral administration of Vit. A. This treatment caused much of the activity of acid phosphatase, beta-glucuronidase and cathepsin to become unsedimentable at 15,000 g in homogenates prepared in 0.25M sucrose. Lysosome-rich fractions of hypervitaminotic livers released beta-glucuronidase and cathepsin more readily into the incubation medium in vitro than did control fractions. These events were accompanied by increases of serum beta-glucuronidase, and are compatible with a direct effect of excess Vit. A on lysosomes.",

author = "G. WEISSMANN and UHR, {J. W.} and L. THOMAS",

note = "Funding Information: Aided by grants from the U.S.P.H.S. and Commission on Immunization, Armed Forces Epidemiological Board. ",

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AB - Acute hypervitaminosis A was induced in guinea pigs by oral administration of Vit. A. This treatment caused much of the activity of acid phosphatase, beta-glucuronidase and cathepsin to become unsedimentable at 15,000 g in homogenates prepared in 0.25M sucrose. Lysosome-rich fractions of hypervitaminotic livers released beta-glucuronidase and cathepsin more readily into the incubation medium in vitro than did control fractions. These events were accompanied by increases of serum beta-glucuronidase, and are compatible with a direct effect of excess Vit. A on lysosomes.

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Acute Hypervitaminosis A in Guinea Pigs. I. Effects on Acid Hydrolases

Abstract

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