Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium

A. M. Colley; M. L. Law; L. A. Drake; Harrison D Cavanagh

doi:10.3109/02713688709025204

Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium

A. M. Colley, M. L. Law, L. A. Drake, Harrison D Cavanagh

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Abstract

Activity of RNA polymerases I, II and III (distinguished using αamanitin) and activity of DNA polymerases α and β (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carbamylcholine-treated and control eyes of rabbits with resurfacing acid burn defects. Incorporation was linear with time for all enzymes up to 30 min. In 10 min assays at varying substrate concentrations, all polymerases from carbamylcholine-treated eyes had significantly elevated Vmax compared to corresponding control enzymes. The drug also increased apparent affinity of RNA polymerase II for UTP and apparent affinity of DNA polymerases a and 3 for dTTP. Results are discussed in relation to potential mechanisms by which effects of carbamylcholine on polymerase activity may be mediated.

Original language	English (US)
Pages (from-to)	477-487
Number of pages	11
Journal	Current Eye Research
Volume	6
Issue number	3
DOIs	https://doi.org/10.3109/02713688709025204
State	Published - 1987

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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@article{cc778a93ec6846a696018777ad837afc,

title = "Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium",

abstract = "Activity of RNA polymerases I, II and III (distinguished using αamanitin) and activity of DNA polymerases α and β (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carbamylcholine-treated and control eyes of rabbits with resurfacing acid burn defects. Incorporation was linear with time for all enzymes up to 30 min. In 10 min assays at varying substrate concentrations, all polymerases from carbamylcholine-treated eyes had significantly elevated Vmax compared to corresponding control enzymes. The drug also increased apparent affinity of RNA polymerase II for UTP and apparent affinity of DNA polymerases a and 3 for dTTP. Results are discussed in relation to potential mechanisms by which effects of carbamylcholine on polymerase activity may be mediated.",

author = "Colley, {A. M.} and Law, {M. L.} and Drake, {L. A.} and Cavanagh, {Harrison D}",

note = "Funding Information: ACRCWLEIXMENTS This work was supported by National Eye Institute Research Grant No. EX04604 and a Departmental grant from Research to Prevent Blindness. The authors gratefully acknowledge the assistance of Mr. Neil Matthews in photography and the administrative and clerical assistance of Mrs. Edith Barron, Mrs. Cynthia Hale, and their secretarial staff.",

year = "1987",

doi = "10.3109/02713688709025204",

language = "English (US)",

volume = "6",

pages = "477--487",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Informa Healthcare",

number = "3",

}

TY - JOUR

T1 - Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium

AU - Colley, A. M.

AU - Law, M. L.

AU - Drake, L. A.

AU - Cavanagh, Harrison D

N1 - Funding Information: ACRCWLEIXMENTS This work was supported by National Eye Institute Research Grant No. EX04604 and a Departmental grant from Research to Prevent Blindness. The authors gratefully acknowledge the assistance of Mr. Neil Matthews in photography and the administrative and clerical assistance of Mrs. Edith Barron, Mrs. Cynthia Hale, and their secretarial staff.

PY - 1987

Y1 - 1987

N2 - Activity of RNA polymerases I, II and III (distinguished using αamanitin) and activity of DNA polymerases α and β (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carbamylcholine-treated and control eyes of rabbits with resurfacing acid burn defects. Incorporation was linear with time for all enzymes up to 30 min. In 10 min assays at varying substrate concentrations, all polymerases from carbamylcholine-treated eyes had significantly elevated Vmax compared to corresponding control enzymes. The drug also increased apparent affinity of RNA polymerase II for UTP and apparent affinity of DNA polymerases a and 3 for dTTP. Results are discussed in relation to potential mechanisms by which effects of carbamylcholine on polymerase activity may be mediated.

AB - Activity of RNA polymerases I, II and III (distinguished using αamanitin) and activity of DNA polymerases α and β (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carbamylcholine-treated and control eyes of rabbits with resurfacing acid burn defects. Incorporation was linear with time for all enzymes up to 30 min. In 10 min assays at varying substrate concentrations, all polymerases from carbamylcholine-treated eyes had significantly elevated Vmax compared to corresponding control enzymes. The drug also increased apparent affinity of RNA polymerase II for UTP and apparent affinity of DNA polymerases a and 3 for dTTP. Results are discussed in relation to potential mechanisms by which effects of carbamylcholine on polymerase activity may be mediated.

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Activity of DNA and RNA polymerases in resurfacing rabbit corneal epithelium

Abstract

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