@article{ed88f96bbb684ebca6c3f6fdc7a28014,
title = "Activation of Skeletal Stem and Progenitor Cells for Bone Regeneration Is Driven by PDGFRβ Signaling",
abstract = "B{\"o}hm et al. show that bone injury activates multiple Osx+-marked cell subsets in the bone and bone marrow environment, representing osteogenesis-competent skeletal stem and progenitor cells (SSPCs). These include PDGFRβ+ SSPCs residing in perivascular niches. The authors found that PDGF-PDGFRβ signaling is functionally required to drive the expansion, recruitment, and blood vessel affinity of reparative SSPCs.",
keywords = "PDGF, PDGF receptor, blood vessels, bone, bone marrow stromal cells, bone regeneration, fracture repair, osteoprogenitors, pericytes, skeletal stem cells",
author = "B{\"o}hm, {Anna Marei} and Naomi Dirckx and Tower, {Robert J.} and Nicolas Peredo and Sebastiaan Vanuytven and Koen Theunis and Elena Nefyodova and Ruben Cardoen and Volkhard Lindner and Thierry Voet and {Van Hul}, Matthias and Christa Maes",
note = "Funding Information: The authors thank D. Rowe, A. McMahon, H.M. Kronenberg, P. Carmeliet, and C. Lo Celso for sharing mouse models. We are grateful to SCEBP members D. Trompet, K. De Samblancx, M. Mesnieres, and G. Belmans for their expertise, support, and helpful discussions, and to the broader SBE with special thanks to F. Luyten, J. Peeters and A. Vandereijcken. A. Cort{\'e}s Calabuig, V. Brys, S. Munck, and N. Corthout are greatly acknowledged for providing expertise on RNA-seq analysis and confocal imaging. We thank H. Kronenberg for critically commenting on the manuscript. This work was funded by grants to C.M. from the European Research Council under the European Union{\textquoteright}s Seventh Framework Programme (FP/2007–2013) (ERC grant agreement no. 282131 ), the Research Fund of the University of Leuven (KU Leuven OT/14/121 ), and the Research Foundation Flanders (FWO, # G0B5219N ). A.-M.B. and M.V.H. were supported by FWO postdoctoral fellowships, N.D. by a doctoral fellowship of the Flemish government Agency for Innovation by Science and Technology (IWT), N.P. by the European Union's Horizon 2020 research and innovation program under Marie Sklodowska-Curie (MSCA-ITN grant agreement no. 721432 ) as Early Stage Researcher, and S.V. by a FWO PhD fellowship for Strategic Basic Research. Funding Information: The authors thank D. Rowe, A. McMahon, H.M. Kronenberg, P. Carmeliet, and C. Lo Celso for sharing mouse models. We are grateful to SCEBP members D. Trompet, K. De Samblancx, M. Mesnieres, and G. Belmans for their expertise, support, and helpful discussions, and to the broader SBE with special thanks to F. Luyten, J. Peeters and A. Vandereijcken. A. Cort?s Calabuig, V. Brys, S. Munck, and N. Corthout are greatly acknowledged for providing expertise on RNA-seq analysis and confocal imaging. We thank H. Kronenberg for critically commenting on the manuscript. This work was funded by grants to C.M. from the European Research Council under the European Union's Seventh Framework Programme (FP/2007?2013) (ERC grant agreement no. 282131), the Research Fund of the University of Leuven (KU Leuven OT/14/121), and the Research Foundation Flanders (FWO, #G0B5219N). A.-M.B. and M.V.H. were supported by FWO postdoctoral fellowships, N.D. by a doctoral fellowship of the Flemish government Agency for Innovation by Science and Technology (IWT), N.P. by the European Union's Horizon 2020 research and innovation program under Marie Sklodowska-Curie (MSCA-ITN grant agreement no. 721432) as Early Stage Researcher, and S.V. by a FWO PhD fellowship for Strategic Basic Research. A.-M.B. N.D. R.J.T. N.P. E.N. R.C. and M.V.H. conducted all main experiments and analyzed data. A.-M.B. S.V. and K.T. performed the scRNA-seq experiment, with expertise and input from T.V. V.L. provided essential materials. All authors participated in designing experiments, interpreting, and discussing results. C.M. conceived the study and supervised the project. A.-M.B. and C.M. wrote the paper. All authors reviewed and edited the manuscript and approved the final version. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = oct,
day = "21",
doi = "10.1016/j.devcel.2019.08.013",
language = "English (US)",
volume = "51",
pages = "236--254.e12",
journal = "Developmental cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "2",
}