Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease

John M. Chase; Steven M. Holland; David E. Greenberg; Kimberly Marshall-Batty; Adrian M. Zelazny; Joseph A. Church

doi:10.1007/s10875-012-9728-6

Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease

John M. Chase, Steven M. Holland, David E. Greenberg, Kimberly Marshall-Batty, Adrian M. Zelazny, Joseph A. Church

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Purpose: Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD. Methods: The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis. Results: Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen's role in the patient's adenitis by showing the patient's specific immune response to the organism. Conclusions: A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.

Original language	English (US)
Pages (from-to)	1193-1196
Number of pages	4
Journal	Journal of Clinical Immunology
Volume	32
Issue number	6
DOIs	https://doi.org/10.1007/s10875-012-9728-6
State	Published - Dec 2012

Keywords

Acidomonas methanolica
Chronic granulomatous disease

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1007/s10875-012-9728-6

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title = "Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease",

abstract = "Purpose: Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD. Methods: The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis. Results: Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen's role in the patient's adenitis by showing the patient's specific immune response to the organism. Conclusions: A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.",

keywords = "Acidomonas methanolica, Chronic granulomatous disease",

author = "Chase, {John M.} and Holland, {Steven M.} and Greenberg, {David E.} and Kimberly Marshall-Batty and Zelazny, {Adrian M.} and Church, {Joseph A.}",

note = "Funding Information: Portions of the patient{\textquoteright}s lymph node were cultured on sheep blood agar, MacConkey agar, chocolate agar, and thioglycolate broth. After 12-day incubation in thioglycolate broth at 35 °C, rare fastidious gram-negative rods grew. Culture containing this rare organism and frozen lymph node material were sent to the Microbiology Service, Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health (NIH) for identification.",

year = "2012",

month = dec,

doi = "10.1007/s10875-012-9728-6",

language = "English (US)",

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AU - Chase, John M.

AU - Holland, Steven M.

AU - Greenberg, David E.

AU - Marshall-Batty, Kimberly

AU - Zelazny, Adrian M.

AU - Church, Joseph A.

N1 - Funding Information: Portions of the patient’s lymph node were cultured on sheep blood agar, MacConkey agar, chocolate agar, and thioglycolate broth. After 12-day incubation in thioglycolate broth at 35 °C, rare fastidious gram-negative rods grew. Culture containing this rare organism and frozen lymph node material were sent to the Microbiology Service, Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health (NIH) for identification.

PY - 2012/12

Y1 - 2012/12

N2 - Purpose: Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD. Methods: The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis. Results: Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen's role in the patient's adenitis by showing the patient's specific immune response to the organism. Conclusions: A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.

AB - Purpose: Adenitis for which no causative organism can be isolated is a common occurrence in patients with chronic granulomatous disease (CGD). Here we identify Acidomonas methanolica as a pathogen associated with adenitis in a patient with CGD. Methods: The causative pathogen was obtained after prolonged incubation of an excised lymph node in thioglycolate broth. Identification was carried out by sequencing the 16s rRNA. Immunoblots were prepared utilizing protein extracts from the case patient's A. methanolica isolate, an ATCC type strain of A. methanolica and G. bethesdensis. Results: Fastidious gram-negative rods grew after prolonged incubation of an excised lymph node in thioglycolate broth. Sequencing of the 16s rRNA identified the organism as A. methanolica. Immunoblot confirmed the pathogen's role in the patient's adenitis by showing the patient's specific immune response to the organism. Conclusions: A. methanolica is the second member of the family, Acetobacteaceae to be associated with adenitis in patients with CGD.

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KW - Chronic granulomatous disease

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Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease

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