TY - JOUR
T1 - Abnormal calcium "sparks" in cardiomyocytes of post-myocardial infarction heart
AU - Huang, Kai
AU - Huang, Dan
AU - Fu, Shengquan
AU - Yang, Chongzhe
AU - Liao, Yuhua
N1 - Funding Information:
Kai HUANG, male, born in 1969, Associate Professor E-mail: huangkai1@yahoo.com #Corresponding author † Dr. Kai HUANG and Dr. Dan HUANG contributed equally to the project. *This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30770881), and a grant from the National Basic Research Program of China (973 Program) (No. 2007CB512000).
PY - 2008/8
Y1 - 2008/8
N2 - In ischemic hypertrophic myocardium, contractile dysfunction can be attributed to the decreased calcium induced calcium release (CICR) in cytoplasm. This study aimed to investigate the electrophysiological properties and the expression of L calcium channel subunits in post-MI myocardium. The ischemic heart remodeling model was established in SD rats. The expressions of calcium channel subunits were determined by realtime RT-PCR. Whole cell patch clamp was used to record the electrophysiological properties of L calcium channel. The results showed that the L calcium channel agonist Bayk 8644 induced the significantly decreased CICR in the rat cardiomyocyte 6 weeks after myocardial infarction (MI). In the post-MI cardiomyocytes, the amplitude of ICaL decreased dramatically and the inactivation curve of the current shifted to more negative potential. At mRNA level, the expression of the calcium channel alpha1c, beta2c subunits decreased dramatically in the ventricle of post-MI rats. The expression of alpha2/delta subunit, however, remained constant. It is concluded that the abnormal expression of the L calcium channel subunits in post-MI cardiomyocytes contributes to the ICaL decrease at early stage of the ischemic remodeling in cardiomyocytes, which leads to the decreased CICR in the cell and contractile dysfunction of myocardium.
AB - In ischemic hypertrophic myocardium, contractile dysfunction can be attributed to the decreased calcium induced calcium release (CICR) in cytoplasm. This study aimed to investigate the electrophysiological properties and the expression of L calcium channel subunits in post-MI myocardium. The ischemic heart remodeling model was established in SD rats. The expressions of calcium channel subunits were determined by realtime RT-PCR. Whole cell patch clamp was used to record the electrophysiological properties of L calcium channel. The results showed that the L calcium channel agonist Bayk 8644 induced the significantly decreased CICR in the rat cardiomyocyte 6 weeks after myocardial infarction (MI). In the post-MI cardiomyocytes, the amplitude of ICaL decreased dramatically and the inactivation curve of the current shifted to more negative potential. At mRNA level, the expression of the calcium channel alpha1c, beta2c subunits decreased dramatically in the ventricle of post-MI rats. The expression of alpha2/delta subunit, however, remained constant. It is concluded that the abnormal expression of the L calcium channel subunits in post-MI cardiomyocytes contributes to the ICaL decrease at early stage of the ischemic remodeling in cardiomyocytes, which leads to the decreased CICR in the cell and contractile dysfunction of myocardium.
KW - Cardiovascular disease
KW - Gene expression
KW - L calcium channel
KW - Myocardial infarction
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U2 - 10.1007/s11596-008-0407-z
DO - 10.1007/s11596-008-0407-z
M3 - Article
C2 - 18704300
AN - SCOPUS:49749123772
SN - 1672-0733
VL - 28
SP - 401
EP - 408
JO - Journal of Huazhong University of Science and Technology - Medical Science
JF - Journal of Huazhong University of Science and Technology - Medical Science
IS - 4
ER -