TY - JOUR
T1 - Ablation of Ventral Midbrain/Pons GABA Neurons Induces Mania-like Behaviors with Altered Sleep Homeostasis and Dopamine D2R-mediated Sleep Reduction
AU - Honda, Takato
AU - Takata, Yohko
AU - Cherasse, Yoan
AU - Mizuno, Seiya
AU - Sugiyama, Fumihiro
AU - Takahashi, Satoru
AU - Funato, Hiromasa
AU - Yanagisawa, Masashi
AU - Lazarus, Michael
AU - Oishi, Yo
N1 - Funding Information:
We thank WPI-IIIS Lazarus/Oishi laboratory and Yanagisawa/Funato laboratory members for suggestions and comments. We also thank M. Sakaguchi, Y. Hayashi, S. Takenawa and K. Kobayashi for their technical advice and support. This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grants 15J06369 (to T.H.), 19K06950 (to Y.T.), 17H02215 (to M.L.) and 18H02534 (to Y.O.); JSPS Overseas Research Fellowship (to T.H.); Takeda Science Foundation (to Y.O.); JST -Mirai Program JPMJMI19D8 (to Y.O.); the Japan Foundation for Applied Enzymology (to Y.O.); the Japan Science and Technology Agency CREST Grant JPMJCR1655 (to M.L. and M.Y.); the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan Grants-in-Aid for Scientific Research on Innovative Areas “WillDynamics” 19H05004 (to M.L.); the World Premier International Research Center Initiative (WPI) from MEXT (to T.H., Y.T., Y.C., S.T., H.F., M.Y., M.L., and Y.O.).
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/6/26
Y1 - 2020/6/26
N2 - Individuals with the neuropsychiatric disorder mania exhibit hyperactivity, elevated mood, and a decreased need for sleep. The brain areas and neuronal populations involved in mania-like behaviors, however, have not been elucidated. In this study, we found that ablating the ventral medial midbrain/pons (VMP) GABAergic neurons induced mania-like behaviors in mice, including hyperactivity, anti-depressive behaviors, reduced anxiety, increased risk-taking behaviors, distractibility, and an extremely shortened sleep time. Strikingly, these mice also showed no rebound sleep after sleep deprivation, suggesting abnormal sleep homeostatic regulation. Dopamine D2 receptor deficiency largely abolished the sleep reduction induced by ablating the VMP GABAergic neurons without affecting the hyperactivity and anti-depressive behaviors. Our data demonstrate that VMP GABAergic neurons are involved in the expression of mania-like behaviors, which can be segregated to the short-sleep and other phenotypes on the basis of the dopamine D2 receptors.
AB - Individuals with the neuropsychiatric disorder mania exhibit hyperactivity, elevated mood, and a decreased need for sleep. The brain areas and neuronal populations involved in mania-like behaviors, however, have not been elucidated. In this study, we found that ablating the ventral medial midbrain/pons (VMP) GABAergic neurons induced mania-like behaviors in mice, including hyperactivity, anti-depressive behaviors, reduced anxiety, increased risk-taking behaviors, distractibility, and an extremely shortened sleep time. Strikingly, these mice also showed no rebound sleep after sleep deprivation, suggesting abnormal sleep homeostatic regulation. Dopamine D2 receptor deficiency largely abolished the sleep reduction induced by ablating the VMP GABAergic neurons without affecting the hyperactivity and anti-depressive behaviors. Our data demonstrate that VMP GABAergic neurons are involved in the expression of mania-like behaviors, which can be segregated to the short-sleep and other phenotypes on the basis of the dopamine D2 receptors.
KW - Behavioral Neuroscience
KW - Cellular Neuroscience
KW - Neuroscience
UR - http://www.scopus.com/inward/record.url?scp=85106794247&partnerID=8YFLogxK
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U2 - 10.1016/j.isci.2020.101240
DO - 10.1016/j.isci.2020.101240
M3 - Article
C2 - 32563157
AN - SCOPUS:85106794247
SN - 2589-0042
VL - 23
JO - iScience
JF - iScience
IS - 6
M1 - 101240
ER -